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Guanylyl Cyclase beta 1 (GUCY1B3) Rabbit pAb (A15276)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Guanylyl Cyclase beta 1 (GUCY1B3) Rabbit pAb (A15276)

Western blot analysis of various lysates using Guanylyl Cyclase beta 1 (GUCY1B3) Rabbit pAb (A15276) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30S.

Overview

Product nameGuanylyl Cyclase beta 1 (GUCY1B3) Rabbit pAb
Catalog No.A15276
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the beta subunit of the soluble guanylate cyclase (sGC), which catalyzes the conversion of GTP (guanosine triphosphate) to cGMP (cyclic guanosine monophosphate). The encoded protein contains an HNOX domain, which serves as a receptor for ligands such as nitric oxide, oxygen and nitrovasodilator drugs. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human Guanylyl Cyclase beta 1 (Guanylyl Cyclase beta 1 (GUCY1B3)) (NP_000848.1).
SequenceMYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPYTFCKAFPFHIIFDRDLVVTQCGNAIYRVLPQLQPGNCSL
Gene ID
Swiss Prot
SynonymsGUCB3; GC-SB3; GUC1B3; GUCSB3; GUCY1B3; GC-S-beta-1; Guanylyl Cyclase beta 1 (GUCY1B3)
Calculated MW71kDa
Observed MW70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse brain, Rat lung, Rat brain
Cellular locationCytoplasm
Customer validation

WB(Macaca mulatta)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Guanylyl Cyclase beta 1 (GUCY1B3) Rabbit pAb (A15276)}

    Western blot - Guanylyl Cyclase beta 1 (GUCY1B3) Rabbit pAb (A15276)

    Western blot analysis of various lysates using Guanylyl Cyclase beta 1 (GUCY1B3) Rabbit pAb (A15276) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30S.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A15276? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    Secondary Antibodies (26)