제품 > 항체 > 다중클론항체(pAb)

HAGH Rabbit pAb (A6615)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HAGH Rabbit pAb (A6615)

Western blot analysis of extracts of various cell lines, using HAGH antibody (A6615) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - HAGH Rabbit pAb (A6615)

Immunofluorescence analysis of A549 cells using HAGH antibody (A6615). Blue: DAPI for nuclear staining.

Overview

Product nameHAGH Rabbit pAb
Catalog No.A6615
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The enzyme encoded by this gene is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. Three transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human HAGH (NP_001035517.1).
SequenceMKVEVLPALTDNYMYLVIDDETKEAAIVDPVQPQKVVDAARKHGVKLTTVLTTHHHWDHAGGNEKLVKLESGLKVYGGDDRIGALTHKITHLSTLQVGSLNVKCLATPCHTSGHICYFVSKPGGSEPPAVFTGDTLFVAGCGKFYEGTADEMCKALLEVLGRLPPDTRVYCGHEYTINNLKFARHVEPGNAAIREKLAWAKEKYSIGEPTVPSTLAEEFTYNPFMRVREKTVQQHAGETDPVTTMRAVRREKDQFKMPRD
Gene ID
Swiss Prot
SynonymsGLO2; GLX2; GLXII; HAGH1
Calculated MW34kDa
Observed MW34kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:10 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMCF7, HepG2, Mouse liver, Mouse kidney, Mouse heart, Rat liver, Rat kidney
Cellular locationCytoplasm, Mitochondrion matrix

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ABclonal:Western blot - HAGH Rabbit pAb (A6615)}

Western blot - HAGH Rabbit pAb (A6615)

Western blot analysis of extracts of various cell lines, using HAGH antibody (A6615) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - HAGH Rabbit pAb (A6615)}

Immunofluorescence - HAGH Rabbit pAb (A6615)

Immunofluorescence analysis of A549 cells using HAGH antibody (A6615). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.