제품 > 항체 > 다중클론항체(pAb)

HCCS Rabbit pAb (A7490)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HCCS Rabbit pAb (A7490)

Western blot analysis of extracts of various cell lines, using HCCS antibody (A7490) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - HCCS Rabbit pAb (A7490)

Immunofluorescence analysis of U-2 OS cells using HCCS Polyclonal Antibody (A7490) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameHCCS Rabbit pAb
Catalog No.A7490
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is an enzyme that covalently links a heme group to the apoprotein of cytochrome c. Defects in this gene are a cause of microphthalmia syndromic type 7 (MCOPS7). Three transcript variants encoding the same protein have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-268 of human HCCS (NP_005324.3).
SequenceMGLSPSAPAVAVQASNASASPPSGCPMHEGKMKGCPVNTEPSGPTCEKKTYSVPAHQERAYEYVECPIRGTAAENKENLDPSNLMPPPNQTPAPDQPFALSTVREESSIPRADSEKKWVYPSEQMFWNAMLKKGWKWKDEDISQKDMYNIIRIHNQNNEQAWKEILKWEALHAAECPCGPSLIRFGGKAKEYSPRARIRSWMGYELPFDRHDWIINRCGTEVRYVIDYYDGGEVNKDYQFTILDVRPALDSLSAVWDRMKVAWWRWTS
Gene ID
Swiss Prot
SynonymsMLS; CCHL; MCOPS7; LSDMCA1
Calculated MW31kDa
Observed MW31kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesSKOV3, MCF7, HeLa, HepG2, SW480, Mouse small intestine, Mouse kidney
Cellular locationLipid-anchor, Membrane, Mitochondrion inner membrane

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ABclonal:Western blot - HCCS Rabbit pAb (A7490)}

Western blot - HCCS Rabbit pAb (A7490)

Western blot analysis of extracts of various cell lines, using HCCS antibody (A7490) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - HCCS Rabbit pAb (A7490)}

Immunofluorescence - HCCS Rabbit pAb (A7490)

Immunofluorescence analysis of U-2 OS cells using HCCS Polyclonal Antibody (A7490) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.