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HCN1 Rabbit pAb (A16198)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - HCN1 Rabbit pAb (A16198)

Western blot analysis of various lysates, using HCN1 Rabbit pAb (A16198) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - HCN1 Rabbit pAb (A16198)

Immunofluorescence analysis of NIH/3T3 cells using HCN1 Rabbit pAb (A16198) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameHCN1 Rabbit pAb
Catalog No.A16198
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The membrane protein encoded by this gene is a hyperpolarization-activated cation channel that contributes to the native pacemaker currents in heart and neurons. The encoded protein can homodimerize or heterodimerize with other pore-forming subunits to form a potassium channel. This channel may act as a receptor for sour tastes.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 791-890 of human HCN1 (NP_066550.2).
SequencePSLPHEVSTLISRPHPTVGESLASIPQPVTAVPGTGLQAGGRSTVPQRVTLFRQMSSGAIPPNRGVPPAPPPPAAALPRESSSVLNTDPDAEKPRFASNL
Gene ID
Swiss Prot
SynonymsBCNG1; DEE24; HAC-2; BCNG-1; EIEE24; GEFSP10; HCN1
Calculated MW99kDa
Observed MW102kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesSH-SY5Y, Neuro-2a
Cellular locationCell membrane, Multi-pass membrane protein.

Documents

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ABclonal:Western blot - HCN1 Rabbit pAb (A16198)}

Western blot - HCN1 Rabbit pAb (A16198)

Western blot analysis of various lysates, using HCN1 Rabbit pAb (A16198) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - HCN1 Rabbit pAb (A16198)}

Immunofluorescence - HCN1 Rabbit pAb (A16198)

Immunofluorescence analysis of NIH/3T3 cells using HCN1 Rabbit pAb (A16198) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

Secondary Antibodies (26)