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HDGFRP3 Rabbit pAb (A8815)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HDGFRP3 Rabbit pAb (A8815)

Western blot analysis of various lysates using HDGFRP3 Rabbit pAb (A8815) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - HDGFRP3 Rabbit pAb (A8815)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using HDGFRP3 Rabbit pAb (A8815) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - HDGFRP3 Rabbit pAb (A8815)

Immunohistochemistry analysis of paraffin-embedded Rat testis using HDGFRP3 Rabbit pAb (A8815) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameHDGFRP3 Rabbit pAb
Catalog No.A8815
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable double-stranded DNA binding activity; microtubule binding activity; and transcription coregulator activity. Predicted to be involved in several processes, including microtubule polymerization; negative regulation of microtubule depolymerization; and neuron projection development. Located in nucleoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 90-203 of human HDGFRP3 (NP_057157.1).
SequenceNNPGVKFTGYQAIQQQSSSETEGEGGNTADASSEEEGDRVEEDGKGKRKNEKAGSKRKKSYTSKKSSKQSRKSPGDEDDKDCKEEENKSSSEGGDAGNDTRNTTSDLQKTSEGT
Gene ID
Swiss Prot
SynonymsHDGF2; HRP-3; HDGF-2; CGI-142; HDGFRP3
Calculated MW22kDa
Observed MW28kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse brain, Mouse kidney, Mouse lung, Rat brain
Cellular locationNucleus

Documents

Certificate of Compliance

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    ABclonal:Western blot - HDGFRP3 Rabbit pAb (A8815)}

    Western blot - HDGFRP3 Rabbit pAb (A8815)

    Western blot analysis of various lysates using HDGFRP3 Rabbit pAb (A8815) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - HDGFRP3 Rabbit pAb (A8815)}

    Immunohistochemistry - HDGFRP3 Rabbit pAb (A8815)

    Immunohistochemistry analysis of paraffin-embedded Rat spleen using HDGFRP3 Rabbit pAb (A8815) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - HDGFRP3 Rabbit pAb (A8815)}

    Immunohistochemistry - HDGFRP3 Rabbit pAb (A8815)

    Immunohistochemistry analysis of paraffin-embedded Rat testis using HDGFRP3 Rabbit pAb (A8815) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.