제품 > 항체 > 다중클론항체(pAb)

HECTD1 Rabbit pAb (A9433)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - HECTD1 Rabbit pAb (A9433)

Western blot analysis of various lysates using HECTD1 Rabbit pAb (A9433) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product nameHECTD1 Rabbit pAb
Catalog No.A9433
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in anatomical structure development; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; and protein K63-linked ubiquitination. Predicted to act upstream of or within several processes, including animal organ development; negative regulation of protein localization to plasma membrane; and protein autoubiquitination.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 2518-2610 of human HECTD1 (NP_056197.3).
SequencePGFLRFVRVLCGMSSDERKAFLQFTTGCSTLPPGGLANLHPRLTVVRKVDATDASYPSVNTCVHYLKLPEYSSEEIMRERLLAATMEKGFHLN
Gene ID
Swiss Prot
SynonymsEULIR; HECTD1
Calculated MW289kDa
Observed MW300kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, U-87MG, LO2
Cellular locationnuclear speck

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    ABclonal:Western blot - HECTD1 Rabbit pAb (A9433)}

    Western blot - HECTD1 Rabbit pAb (A9433)

    Western blot analysis of various lysates using HECTD1 Rabbit pAb (A9433) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.