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HEXA Rabbit pAb (A5646)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HEXA Rabbit pAb (A5646)

Western blot analysis of various lysates using HEXA Rabbit pAb (A5646) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - HEXA Rabbit pAb (A5646)

Western blot analysis of lysates from Rat kidney using HEXA Rabbit pAb (A5646) at 1:500 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - HEXA Rabbit pAb (A5646)

Immunohistochemistry analysis of paraffin-embedded Rat brain using HEXA Rabbit pAb (A5646) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - HEXA Rabbit pAb (A5646)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using HEXA Rabbit pAb (A5646) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - HEXA Rabbit pAb (A5646)

Immunofluorescence analysis of paraffin-embedded Rat kidney tissue using HEXA Rabbit pAb(A5646) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - HEXA Rabbit pAb (A5646)

Immunofluorescence analysis of paraffin-embedded Human kidney tissue using HEXA Rabbit pAb(A5646) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameHEXA Rabbit pAb
Catalog No.A5646
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the glycosyl hydrolase 20 family of proteins. The encoded preproprotein is proteolytically processed to generate the alpha subunit of the lysosomal enzyme beta-hexosaminidase. This enzyme, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Mutations in this gene lead to an accumulation of GM2 ganglioside in neurons, the underlying cause of neurodegenerative disorders termed the GM2 gangliosidoses, including Tay-Sachs disease (GM2-gangliosidosis type I). Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 270-529 of human HEXA (NP_000511.2).
SequenceIPGLLTPCYSGSEPSGTFGPVNPSLNNTYEFMSTFFLEVSSVFPDFYLHLGGDEVDFTCWKSNPEIQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYGKGYVVWQEVFDNKVKIQPDTIIQVWREDIPVNYMKELELVTKAGFRALLSAPWYLNRISYGPDWKDFYIVEPLAFEGTPEQKALVIGGEACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKLTSDLTFAYERLSHFRCELLRRGVQAQPLNVGFCEQEFEQT
Gene ID
Swiss Prot
SynonymsTSD; HEXA
Calculated MW61kDa
Observed MW55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2, A-549, HeLa, Mouse liver, Mouse testis, Rat testis, Mouse kidney, Rat kidney
Cellular locationLysosome.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - HEXA Rabbit pAb (A5646)}

    Western blot - HEXA Rabbit pAb (A5646)

    Western blot analysis of various lysates using HEXA Rabbit pAb (A5646) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - HEXA Rabbit pAb (A5646)}

    Western blot - HEXA Rabbit pAb (A5646)

    Western blot analysis of lysates from Rat kidney using HEXA Rabbit pAb (A5646) at 1:500 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - HEXA Rabbit pAb (A5646)}

    Immunohistochemistry - HEXA Rabbit pAb (A5646)

    Immunohistochemistry analysis of paraffin-embedded Rat brain using HEXA Rabbit pAb (A5646) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - HEXA Rabbit pAb (A5646)}

    Immunohistochemistry - HEXA Rabbit pAb (A5646)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney using HEXA Rabbit pAb (A5646) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunofluorescence - HEXA Rabbit pAb (A5646)}

    Immunofluorescence - HEXA Rabbit pAb (A5646)

    Immunofluorescence analysis of paraffin-embedded Rat kidney tissue using HEXA Rabbit pAb(A5646) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
    ABclonal:Immunofluorescence - HEXA Rabbit pAb (A5646)}

    Immunofluorescence - HEXA Rabbit pAb (A5646)

    Immunofluorescence analysis of paraffin-embedded Human kidney tissue using HEXA Rabbit pAb(A5646) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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