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HFM1 Rabbit pAb (A8600)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HFM1 Rabbit pAb (A8600)

Western blot analysis of lysates from K-562 cells, using HFM1 Rabbit pAb (A8600) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - HFM1 Rabbit pAb (A8600)

Immunohistochemistry analysis of paraffin-embedded Rat testis using HFM1 Rabbit pAb (A8600) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - HFM1 Rabbit pAb (A8600)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using HFM1 Rabbit pAb (A8600) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameHFM1 Rabbit pAb
Catalog No.A8600
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is thought to be an ATP-dependent DNA helicase and is expressed mainly in germ-line cells. Defects in this gene are a cause of premature ovarian failure 9 (POF9).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HFM1 (NP_001017975.4).
SequenceMLKSNDCLFSLENLFFEKPDEVENHPDNEKSLDWFLPPAPLISEIPDTQELEEELESHKLLGQEKRPKMLTSNLKITNEDTNYISLTQKFQFAFPSDKYEQDDLNLEGVGNNDLSHVAGKLTYASQKYKNHIGTEIAPEKSVPDDTKLVNFAEDKGESTSVFRKRLFKISDNIHGSAYSNDNELDSHIGSVKIVQTEMNKGKSRNYSNSKQKFQYSANVFTANNAFSASEIGEGMFKAPS
Gene ID
Swiss Prot
SynonymsMER3; POF9; Si-11; SEC63D1; Si-11-6; helicase; HFM1
Calculated MW163kDa
Observed MW130kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesK-562
Cellular locationnucleus

Documents

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    ABclonal:Western blot - HFM1 Rabbit pAb (A8600)}

    Western blot - HFM1 Rabbit pAb (A8600)

    Western blot analysis of lysates from K-562 cells, using HFM1 Rabbit pAb (A8600) at 1:500 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - HFM1 Rabbit pAb (A8600)}

    Immunohistochemistry - HFM1 Rabbit pAb (A8600)

    Immunohistochemistry analysis of paraffin-embedded Rat testis using HFM1 Rabbit pAb (A8600) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - HFM1 Rabbit pAb (A8600)}

    Immunohistochemistry - HFM1 Rabbit pAb (A8600)

    Immunohistochemistry analysis of paraffin-embedded Mouse testis using HFM1 Rabbit pAb (A8600) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.