제품 > 항체 > 다중클론항체(pAb)

HIGD1A Rabbit pAb (A14582)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - HIGD1A Rabbit pAb (A14582)

Western blot analysis of various lysates using HIGD1A Rabbit pAb (A14582) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - HIGD1A Rabbit pAb (A14582)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using HIGD1A Rabbit pAb (A14582) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - HIGD1A Rabbit pAb (A14582)

Immunofluorescence analysis of U-2 OS cells using HIGD1A Rabbit pAb (A14582) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameHIGD1A Rabbit pAb
Catalog No.A14582
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Acts upstream of or within negative regulation of apoptotic process. Located in mitochondrion and nucleoplasm. Part of protein-containing complex.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-93 of human HIGD1A (NP_054775.2).
SequenceMSTDTGVSLPSYEEDQGSKLIRKAKEAPFVPVGIAGFAAIVAYGLYKLKSRGNTKMSIHLIHMRVAAQGFVVGAMTVGMGYSMYREFWAKPKP
Gene ID
Swiss Prot
SynonymsHIG1; RCF1a; HIGD1A
Calculated MW10kDa
Observed MW14kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, HepG2, 293T, K-562
Cellular locationMitochondrion inner membrane, Mitochondrion membrane, Multi-pass membrane protein

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - HIGD1A Rabbit pAb (A14582)}

    Western blot - HIGD1A Rabbit pAb (A14582)

    Western blot analysis of various lysates using HIGD1A Rabbit pAb (A14582) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - HIGD1A Rabbit pAb (A14582)}

    Immunohistochemistry - HIGD1A Rabbit pAb (A14582)

    Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using HIGD1A Rabbit pAb (A14582) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - HIGD1A Rabbit pAb (A14582)}

    Immunofluorescence - HIGD1A Rabbit pAb (A14582)

    Immunofluorescence analysis of U-2 OS cells using HIGD1A Rabbit pAb (A14582) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)