제품 > 항체 > 다중클론항체(pAb)

HIP1R Rabbit pAb (A12026)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HIP1R Rabbit pAb (A12026)

Western blot analysis of various lysates using HIP1R Rabbit pAb (A12026) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameHIP1R Rabbit pAb
Catalog No.A12026
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables several functions, including phosphatidylinositol phosphate binding activity; phosphatidylinositol-3, 4-bisphosphate binding activity; and protein homodimerization activity. Involved in several processes, including positive regulation of signal transduction; protein stabilization; and regulation of organelle organization. Located in clathrin-coated vesicle; cytosol; and ruffle membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human HIP1R (NP_003950.1).
SequenceKPVVVIPEEAPEDEEPENLIEISTGPPAGEPVVVADLFDQTFGPPNGSVKDDRDLQIESLKREVEMLRSELEKIKLEAQRYIAQLKSQVNALEGELEEQRKQKQKALVDNEQLRHELAQLRAAQLEGERSQGLREEAERKASATEARYNKLKEKHSELVHVHAELLRKNADTAKQLTVTQQSQEEVARVKEQLAFQVEQVKRESELKLEEKSDQLEKLKRELEAKAGELARAQEALSHTEQSKSELSSRLDTLSAEKDALSGAVRQREADLLAAQSLVRETEAALSREQQRSSQEQGELQG
Gene ID
Swiss Prot
SynonymsHIP3; HIP12; ILWEQ; HIP1R
Calculated MW119kDa
Observed MW130kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesU-87MG, 293T, HeLa, Mouse brain, Mouse spleen, Mouse liver, Rat brain
Cellular locationCytoplasm, Cytoplasmic vesicle, Endomembrane system, clathrin-coated vesicle membrane, perinuclear region

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    ABclonal:Western blot - HIP1R Rabbit pAb (A12026)}

    Western blot - HIP1R Rabbit pAb (A12026)

    Western blot analysis of various lysates using HIP1R Rabbit pAb (A12026) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.