Western blot - HLA-DPB1 Rabbit pAb (A17495)
Western blot analysis of various lysates using HLA-DPB1 Rabbit pAb (A17495) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot - HLA-DPB1 Rabbit pAb (A17495)
Western blot analysis of various lysates using HLA-DPB1 Rabbit pAb (A17495) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
Immunohistochemistry - HLA-DPB1 Rabbit pAb (A17495)
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using HLA-DPB1 Rabbit pAb (A17495) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - HLA-DPB1 Rabbit pAb (A17495)
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using HLA-DPB1 Rabbit pAb (A17495) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - HLA-DPB1 Rabbit pAb (A17495)
Immunohistochemistry analysis of paraffin-embedded Rat spleen using HLA-DPB1 Rabbit pAb (A17495) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry - HLA-DPB1 Rabbit pAb (A17495)
Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using HLA-DPB1 Rabbit pAb (A17495) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry - HLA-DPB1 Rabbit pAb (A17495)
Immunohistochemistry analysis of paraffin-embedded Human lung tissue using HLA-DPB1 Rabbit pAb (A17495) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence - HLA-DPB1 Rabbit pAb (A17495)
Immunofluorescence analysis of C6 cells using HLA-DPB1 Rabbit pAb (A17495) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - HLA-DPB1 Rabbit pAb (A17495)
Immunofluorescence analysis of HeLa cells using HLA-DPB1 Rabbit pAb (A17495) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - HLA-DPB1 Rabbit pAb (A17495)
Immunofluorescence analysis of NIH/3T3 cells using HLA-DPB1 Rabbit pAb (A17495) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - HLA-DPB1 Rabbit pAb (A17495)
Immunofluorescence analysis of Raji cells using HLA-DPB1 Rabbit pAb (A17495) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.