제품 > 항체 > 다중클론항체(pAb)

HRSP12 Rabbit pAb (A4392)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HRSP12 Rabbit pAb (A4392)

Western blot analysis of extracts of various cell lines, using HRSP12 antibody (A4392) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameHRSP12 Rabbit pAb
Catalog No.A4392
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables 2-iminobutanoate deaminase activity and mRNA binding activity. Involved in mRNA catabolic process; mRNA destabilization; and organonitrogen compound catabolic process. Located in cytoplasm and nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-137 of human HRSP12 (NP_005827.1).
SequenceMSSLIRRVISTAKAPGAIGPYSQAVLVDRTIYISGQIGMDPSSGQLVSGGVAEEAKQALKNMGEILKAAGCDFTNVVKTTVLLADINDFNTVNEIYKQYFKSNFPARAAYQVAALPKGSRIEIEAVAIQGPLTTASL
Gene ID
Swiss Prot
SynonymsPSP; P14.5; UK114; HRSP12; hp14.5
Calculated MW14kDa
Observed MW14kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMCF7, K-562, Mouse liver, Mouse kidney, Rat kidney
Cellular locationCytoplasm, Nucleus

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ABclonal:Western blot - HRSP12 Rabbit pAb (A4392)}

Western blot - HRSP12 Rabbit pAb (A4392)

Western blot analysis of extracts of various cell lines, using HRSP12 antibody (A4392) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)