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HS1BP3 Rabbit pAb (A8250)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HS1BP3 Rabbit pAb (A8250)

Western blot analysis of extracts of various cell lines, using HS1BP3 antibody (A8250) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

Overview

Product nameHS1BP3 Rabbit pAb
Catalog No.A8250
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene shares similarity with mouse Hs1bp3, an Hcls1/Hs1-interacting protein that may be involved in lymphocyte activation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human HS1BP3 (NP_071905.3).
SequenceMQSPAVLVTSRRLQNAHTGLDLTVPQHQEVRGKMMSGHVEYQILVVTRLAAFKSAKHRPEDVVQFLVSKKYSEIEEFYQKLSSRYAAASLPPLPRKVLFVGESDIRERRAVFNEILRCVSKDAELAGSPELLEFLGTRSPGAAGLTSRDSSVLDGTDSQTGNDEEAFDFFEEQDQVAEEGPPVQSLKGEDAEESLEEEEA
Gene ID
Swiss Prot
SynonymsETM2; HS1-BP3
Calculated MW43kDa
Observed MW55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHepG2, Mouse liver, Mouse kidney, Mouse heart, Rat liver
Cellular locationendoplasmic reticulum, mitochondrion

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    ABclonal:Western blot - HS1BP3 Rabbit pAb (A8250)}

    Western blot - HS1BP3 Rabbit pAb (A8250)

    Western blot analysis of extracts of various cell lines, using HS1BP3 antibody (A8250) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 15s.

    * For research use only. Not for therapeutic or diagnostic purposes.