제품 > 항체 > 다중클론항체(pAb)

HSD17B7 Rabbit pAb (A17157)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - HSD17B7 Rabbit pAb (A17157)

Western blot analysis of various lysates using HSD17B7 Rabbit pAb (A17157) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5min.

ABclonal:Immunohistochemistry - HSD17B7 Rabbit pAb (A17157)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using HSD17B7 Rabbit pAb (A17157) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product nameHSD17B7 Rabbit pAb
Catalog No.A17157
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
HSD17B7 encodes an enzyme that functions both as a 17-beta-hydroxysteroid dehydrogenase (EC 1.1.1.62) in the biosynthesis of sex steroids and as a 3-ketosteroid reductase (EC 1.1.1.270) in the biosynthesis of cholesterol (Marijanovic et al., 2003 [PubMed 12829805]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 110-200 of human HSD17B7 (NP_057455.1).
SequenceSRKVIHMFSTAEGLLTQGDKITADGLQEVFETNVFGHFILIRELEPLLCHSDNPSQLIWTSSRSARKSNFSLEDFQHSKGKEPYSSSKYAT
Gene ID
Swiss Prot
SynonymsPRAP; SDR37C1; HSD17B7
Calculated MW38kDa
Observed MW38kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse liver, Mouse ovary
Cellular locationCell membrane, Single-pass membrane protein

Documents

Certificate of Compliance

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    ABclonal:Western blot - HSD17B7 Rabbit pAb (A17157)}

    Western blot - HSD17B7 Rabbit pAb (A17157)

    Western blot analysis of various lysates using HSD17B7 Rabbit pAb (A17157) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5min.
    ABclonal:Immunohistochemistry - HSD17B7 Rabbit pAb (A17157)}

    Immunohistochemistry - HSD17B7 Rabbit pAb (A17157)

    Immunohistochemistry analysis of paraffin-embedded Human breast cancer using HSD17B7 Rabbit pAb (A17157) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.