제품 > 항체 > 다중클론항체(pAb)

HSD17B8 Rabbit pAb (A12947)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - HSD17B8 Rabbit pAb (A12947)

Western blot analysis of various lysates using HSD17B8 Rabbit pAb (A12947) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - HSD17B8 Rabbit pAb (A12947)

Immunofluorescence analysis of U-2 OS cells using HSD17B8 Rabbit pAb (A12947) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameHSD17B8 Rabbit pAb
Catalog No.A12947
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. The protein encoded by this gene is similar to Ke6 and is a member of the short-chain dehydrogenase superfamily. An alternatively spliced transcript of this gene has been detected, but the full-length nature of this variant has not been determined.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human HSD17B8 (NP_055049.1).
SequenceMASQLQNRLRSALALVTGAGSGIGRAVSVRLAGEGATVAACDLDRAAAQETVRLLGGPGSKEGPPRGNHAAFQADVSEARAARCLLEQVQACFSRPPSVVVSCAGITQDEFLLHMSEDDWDKVIAVNLKGTFLVTQAAAQALVSNGCRGSIINISSIVGKVGNVGQTNYAASKAGVIGLTQTAARELGRHGIRCNSVLPGFIATPMTQKVPQKVVDKITEMIPMGHLGDPEDVADVVAFLASEDSGYITGTSVEVTGGLFM
Gene ID
Swiss Prot
SynonymsKE6; FABG; HKE6; FABGL; RING2; H2-KE6; SDR30C1; D6S2245E; dJ1033B10.9; HSD17B8
Calculated MW27kDa
Observed MW27kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHepG2, BxPC-3, U-87MG, HeLa, Mouse liver, Mouse pancreas, Rat heart
Cellular locationMitochondrion matrix

Documents

Certificate of Compliance

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    ABclonal:Western blot - HSD17B8 Rabbit pAb (A12947)}

    Western blot - HSD17B8 Rabbit pAb (A12947)

    Western blot analysis of various lysates using HSD17B8 Rabbit pAb (A12947) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - HSD17B8 Rabbit pAb (A12947)}

    Immunofluorescence - HSD17B8 Rabbit pAb (A12947)

    Immunofluorescence analysis of U-2 OS cells using HSD17B8 Rabbit pAb (A12947) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.