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HSP20/HSPB6 Rabbit pAb (A9887)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - HSP20/HSPB6 Rabbit pAb (A9887)

Western blot analysis of extracts of mouse lung, using HSP20/HSP20/HSPB6 antibody (A9887) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameHSP20/HSPB6 Rabbit pAb
Catalog No.A9887
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This locus encodes a heat shock protein. The encoded protein likely plays a role in smooth muscle relaxation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human HSP20/HSP20/HSPB6 (NP_653218.1).
SequenceMEIPVPVQPSWLRRASAPLPGLSAPGRLFDQRFGEGLLEAELAALCPTTLAPYYLRAPSVALPVAQVPTDPGHFSVLLDVKHFSPEEIAVKVVGEHVEVHARHEERPDEHGFVAREFHRRYRLPPGVDPAAVTSALSPEGVLSIQAAPASAQAPPPAAAK
Gene ID
Swiss Prot
SynonymsHEL55; Hsp20; PPP1R91
Calculated MW17kDa
Observed MW17kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse lung
Cellular locationCytoplasm, Nucleus

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    ABclonal:Western blot - HSP20/HSPB6 Rabbit pAb (A9887)}

    Western blot - HSP20/HSPB6 Rabbit pAb (A9887)

    Western blot analysis of extracts of mouse lung, using HSP20/HSP20/HSPB6 antibody (A9887) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)