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HSP70 Rabbit mAb (A23457)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Wheat

ABclonal:Western blot - HSP70 Rabbit mAb (A23457)

Western blot analysis of various lysates, using HSP70 Rabbit mAb (A23457) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)..
Exposure time: 30s.

ABclonal:Western blot - HSP70 Rabbit mAb (A23457)

Western blot analysis of lysates from Wheat using HSP70 Rabbit mAb (A23457) at 1:95000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Immunohistochemistry - HSP70 Rabbit mAb (A23457)

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using HSP70 Rabbit mAb (A23457) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HSP70 Rabbit mAb (A23457)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using HSP70 Rabbit mAb (A23457) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HSP70 Rabbit mAb (A23457)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using HSP70 Rabbit mAb (A23457) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HSP70 Rabbit mAb (A23457)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using HSP70 Rabbit mAb (A23457) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - HSP70 Rabbit mAb (A23457)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using HSP70 Rabbit mAb (A23457) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - HSP70 Rabbit mAb (A23457)

Confocal imaging of HeLa cells using HSP70 Rabbit mAb (A4777, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

Overview

Product nameHSP70 Rabbit mAb
Catalog No.A23457
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC53082
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 500-600 of human HSP70. (NP_005336.3).
SequenceKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELE
Gene ID
Swiss Prot
SynonymsHSP70; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-2; HSP70.1; HSP70.2; HSP70-1A; HEL-S-103
Calculated MW70kDa
Observed MW70kDa
ReactivityHuman, Mouse, Rat, Wheat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:40000
  • IHC-P 1:1000 - 1:5000
  • IF/ICC 1:100 - 1:400
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain, Rat liver, Wheat
Cellular location
Customer validation

WB(Sus scrofa)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - HSP70 Rabbit mAb (A23457)}

    Western blot - HSP70 Rabbit mAb (A23457)

    Western blot analysis of various lysates, using HSP70 Rabbit mAb (A23457) at 1:10000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020)..
    Exposure time: 30s.
    ABclonal:Western blot - HSP70 Rabbit mAb (A23457)}

    Western blot - HSP70 Rabbit mAb (A23457)

    Western blot analysis of lysates from Wheat using HSP70 Rabbit mAb (A23457) at 1:95000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 45s.
    ABclonal:Immunohistochemistry - HSP70 Rabbit mAb (A23457)}

    Immunohistochemistry - HSP70 Rabbit mAb (A23457)

    Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using HSP70 Rabbit mAb (A23457) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HSP70 Rabbit mAb (A23457)}

    Immunohistochemistry - HSP70 Rabbit mAb (A23457)

    Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using HSP70 Rabbit mAb (A23457) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HSP70 Rabbit mAb (A23457)}

    Immunohistochemistry - HSP70 Rabbit mAb (A23457)

    Immunohistochemistry analysis of paraffin-embedded Human liver tissue using HSP70 Rabbit mAb (A23457) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HSP70 Rabbit mAb (A23457)}

    Immunohistochemistry - HSP70 Rabbit mAb (A23457)

    Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using HSP70 Rabbit mAb (A23457) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - HSP70 Rabbit mAb (A23457)}

    Immunohistochemistry - HSP70 Rabbit mAb (A23457)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using HSP70 Rabbit mAb (A23457) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - HSP70 Rabbit mAb (A23457)}

    Immunofluorescence - HSP70 Rabbit mAb (A23457)

    Confocal imaging of HeLa cells using HSP70 Rabbit mAb (A4777, dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A23457? Please let us know so that we can cite the reference in this datasheet.

    항체 (2)

    Secondary Antibodies (25)