제품 > 항체 > 다중클론항체(pAb)

HUS1 Rabbit pAb (A5407)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using HUS1 Rabbit pAb (A5407) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - HUS1 Rabbit pAb (A5407)

Immunofluorescence analysis of U2OS cells using HUS1 Rabbit pAb (A5407). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - HUS1 Rabbit pAb (A5407)

Immunoprecipitation analysis of 200 μg extracts of 293T cells using 1 μg HUS1 antibody (A5407). Western blot was performed from the immunoprecipitate using HUS1 antibody (A5407) at a dilution of 1:1000.

Overview

Product name	HUS1 Rabbit pAb
Catalog No.	A5407
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HUS1 (NP_004498.1).
Sequence	MKFRAKIVDGACLNHFTRISNMIAKLAKTCTLRISPDKLNFILCDKLANGGVSMWCELEQENFFNEFQMEGVSAENNEIYLELTSENLSRALKTAQNARALKIKLTNKHFPCLTVSVELLSMSSSSRIVTHDIPIKVIPRKLWKDLQEPVVPDPDVSIYLPVLKTMKSVVEKMKNISNHLVIEANLDGELNLKIETELVCVTTHFKDLGNPPLASESTHEDRNVEHMAEVHIDIRKLLQFLAGQQVNPTKALCNIVNNKMVHFDLLHEDVSLQYFIPALS
Gene ID
Swiss Prot
Synonyms	hHUS1; HUS1
Calculated MW	32kDa
Observed MW	35kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:100 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	OVCAR-3, K-562, 293T
Cellular location	Cytoplasm, Nucleus
Customer validation	WB(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

연구후성유전학 & 핵 신호전달DNA 손상 및 복구

HUS1 Rabbit pAb images

Western blot - HUS1 Rabbit pAb (A5407)

Immunofluorescence - HUS1 Rabbit pAb (A5407)

Immunofluorescence analysis of U2OS cells using HUS1 Rabbit pAb (A5407). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence - HUS1 Rabbit pAb (A5407)

Immunofluorescence analysis of U2OS cells using HUS1 Rabbit pAb (A5407). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunoprecipitation - HUS1 Rabbit pAb (A5407)

* For research use only. Not for therapeutic or diagnostic purposes.