제품 > 항체 > 다중클론항체(pAb)

Hd1 Rabbit pAb (A20268)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - Hd1  Rabbit pAb (A20268)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using Hd1 Rabbit pAb (A20268) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.

Overview

Product nameHd1 Rabbit pAb
Catalog No.A20268
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Probable transcription factor involved in the regulation of flower development. Required for the promotion of flowering under short day (SD) conditions and the suppression of flowering under long day (LD) conditions. Regulates positively the floral activator HEADING DATE 3a (HD3A) under SD and negatively under LD conditions. The GIGANTEA-CONSTANS-FLOWER LOCUS T (GI-CO-FT) pathway to control photoperiodic flowering under LD is conserved between Arabidopsis and rice, but the regulation of the downstream gene by the upstream regulatory gene is reversed in the two species. In Arabidopsis, GI acts as an activator of CO, which in turn activates the floral activator FT under LD conditions. In rice, GI activates HD1/CO in a similar manner to that in Arabidopsis. However, under LD conditions, HD1 suppresses HD3A/FT expression, causing the suppression of flowering.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-320 of Oryza sativa Hd1 . (Q9FDX8).
SequenceMNYNFGGNVFDQEVGVGGEGGGGGEGSGCPWARPCDGCRAAPSVVYCRADAAYLCASCDARVHAANRVASRHERVRVCEACERAPAALACRADAAALCVACDVQVHSANPLPAITIPATSVLAEAVVATATVLGDKDEEVDSWLLLSKDSDNNNNNNNNNDNDNNDNNNSNSSNNGMYFGEVDEYFDLVGYNSYYDNRIENNQDRQYGMHEQQEQQQQQQEMQKEFAEKEGSECVVPSQITMLSEQQHSGYGVVGADQAASMTAGVSAYTDSISNSISFSSMEAGIVPDSTVIDMPNSRILTPAGAINLFSGPSLQMSLH
Gene ID
Swiss Prot
SynonymsHd1; SE1; OsHd1; Hd1
Calculated MW43kDa
Observed MW50kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesstems (before flower), leaves sheath (before flower), flag leaves (after flower)
Cellular locationnucleus
Customer validation

WB(Oryza sativa)

    ABclonal:Western blot - Hd1  Rabbit pAb (A20268)}

    Western blot - Hd1 Rabbit pAb (A20268)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using Hd1 Rabbit pAb (A20268) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 120s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A20268? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)