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Hemopexin (HPX) Rabbit mAb (A9133)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Hemopexin (HPX) Rabbit mAb (A9133)

Western blot analysis of lysates from Mouse lung, using Hemopexin (HPX) (HPX) Rabbit mAb (A9133) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 2min.

ABclonal:Western blot - Hemopexin (HPX) Rabbit mAb (A9133)

Western blot analysis of lysates from Human serum, using Hemopexin (HPX) (HPX) Rabbit mAb (A9133) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Hemopexin (HPX) Rabbit mAb (A9133)

Immunohistochemistry analysis of paraffin-embedded Rat liver using Hemopexin (HPX) (HPX) Rabbit mAb (A9133) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Hemopexin (HPX) Rabbit mAb (A9133)

Confocal imaging of paraffin-embedded Rat liver using Hemopexin (HPX) Rabbit mAb (A9133, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - Hemopexin (HPX) Rabbit mAb (A9133)

Confocal imaging of paraffin-embedded Human liver using Hemopexin (HPX) Rabbit mAb (A9133, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameHemopexin (HPX) Rabbit mAb
Catalog No.A9133
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1444
This gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 363-462 of human Hemopexin (HPX) (P02790).
SequenceAFICPGSSRLHIMAGRRLWWLDLKSGAQATWTELPWPHEKVDGALCMEKSLGPNSCSANGPGLYLIHGPNLYCYSDVEKLNAAKALPQPQNVTSLLGCTH
Gene ID
Swiss Prot
SynonymsHX; Hemopexin (HPX)
Calculated MW52kDa
Observed MW70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHuman serum, Mouse lung
Cellular locationSecreted

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ABclonal:Western blot - Hemopexin (HPX) Rabbit mAb (A9133)}

Western blot - Hemopexin (HPX) Rabbit mAb (A9133)

Western blot analysis of lysates from Mouse lung, using Hemopexin (HPX) (HPX) Rabbit mAb (A9133) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 2min.
ABclonal:Western blot - Hemopexin (HPX) Rabbit mAb (A9133)}

Western blot - Hemopexin (HPX) Rabbit mAb (A9133)

Western blot analysis of lysates from Human serum, using Hemopexin (HPX) (HPX) Rabbit mAb (A9133) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Hemopexin (HPX) Rabbit mAb (A9133)}

Immunohistochemistry - Hemopexin (HPX) Rabbit mAb (A9133)

Immunohistochemistry analysis of paraffin-embedded Rat liver using Hemopexin (HPX) (HPX) Rabbit mAb (A9133) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Hemopexin (HPX) Rabbit mAb (A9133)}

Immunofluorescence - Hemopexin (HPX) Rabbit mAb (A9133)

Confocal imaging of paraffin-embedded Rat liver using Hemopexin (HPX) Rabbit mAb (A9133, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - Hemopexin (HPX) Rabbit mAb (A9133)}

Immunofluorescence - Hemopexin (HPX) Rabbit mAb (A9133)

Confocal imaging of paraffin-embedded Human liver using Hemopexin (HPX) Rabbit mAb (A9133, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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