제품 > 항체 > 다중클론항체(pAb)

Hemopexin (HPX) Rabbit pAb (A5603)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Hemopexin (HPX) Rabbit pAb (A5603)

Western blot analysis of extracts of various cell lines, using Hemopexin (Hemopexin (HPX)) antibody (A5603) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameHemopexin (HPX) Rabbit pAb
Catalog No.A5603
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 24-245 of human Hemopexin (Hemopexin (HPX)) (NP_000604.1).
SequenceTPLPPTSAHGNVAEGETKPDPDVTERCSDGWSFDATTLDDNGTMLFFKGEFVWKSHKWDRELISERWKNFPSPVDAAFRQGHNSVFLIKGDKVWVYPPEKKEKGYPKLLQDEFPGIPSPLDAAVECHRGECQAEGVLFFQGDREWFWDLATGTMKERSWPAVGNCSSALRWLGRYYCFQGNQFLRFDPVRGEVPPRYPRDVRDYFMPCPGRGHGHRNGTGHG
Gene ID
Swiss Prot
SynonymsHX
Calculated MW52kDa
Observed MW68kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHL-60, HepG2, BT-474, Mouse liver, Mouse pancreas
Cellular locationSecreted
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - Hemopexin (HPX) Rabbit pAb (A5603)}

Western blot - Hemopexin (HPX) Rabbit pAb (A5603)

Western blot analysis of extracts of various cell lines, using Hemopexin (Hemopexin (HPX)) antibody (A5603) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A5603? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)