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Hippocalcin (HPCA) Rabbit mAb (A19696)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Hippocalcin (HPCA) Rabbit mAb (A19696)

Western blot analysis of extracts of various cell lines, using Hippocalcin (HPCA) (HPCA) Rabbit mAb (A19696) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameHippocalcin (HPCA) Rabbit mAb
Catalog No.A19696
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2235
The protein encoded by this gene is a member of neuron-specific calcium-binding proteins family found in the retina and brain. This protein is associated with the plasma membrane. It has similarities to proteins located in the photoreceptor cells that regulate photosignal transduction in a calcium-sensitive manner. This protein displays recoverin activity and a calcium-dependent inhibition of rhodopsin kinase. It is identical to the rat and mouse hippocalcin proteins and thought to play an important role in neurons of the central nervous system in a number of species.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Hippocalcin (HPCA) (P84074).
SequenceMGKQNSKLRPEMLQDLRENTEFSELELQEWYKGFLKDCPTGILNVDEFKKIYANFFPYGDASKFAEHVFRTFDTNSDGTIDFREFIIALSVTSRGRLEQK
Gene ID
Swiss Prot
SynonymsBDR2; DYT2
Calculated MW22kDa
Observed MW22kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesPC-12, MCF7, C6, Mouse testis, Rat brain
Cellular locationaxon, cytoplasm, cytosol, dendrite cytoplasm, dendrite membrane, dendritic spine head, glutamatergic synapse, perikaryon

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ABclonal:Western blot - Hippocalcin (HPCA) Rabbit mAb (A19696)}

Western blot - Hippocalcin (HPCA) Rabbit mAb (A19696)

Western blot analysis of extracts of various cell lines, using Hippocalcin (HPCA) (HPCA) Rabbit mAb (A19696) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.