Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat
Western blot analysis of various lysates using Homer1 Rabbit mAb (A21136) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
| Product name | Homer1 Rabbit mAb |
|---|---|
| Catalog No. | A21136 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC3008 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 101-220 of human HOMER1 (NP_004263.1). |
|---|---|
| Sequence | RLAKEKSQEKMELTSTPSQESAGGDLQSPLTPESINGTDDERTPDVTQNSEPRAEPTQNALPFSHSSAISKHWEAELATLKGNNAKLTAALLESTANVKQWKQQLAAYQE |
| Gene ID | |
| Swiss Prot | |
| Synonyms | HOMER; SYN47; Ves-1; HOMER1A; HOMER1B; HOMER1C; Homer1 |
| Calculated MW | 40kDa |
| Observed MW | 46kDa |
| Reactivity | Human, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | U-87MG, Rat skeletal muscle |
| Cellular location | Cell junction, Cytoplasm, postsynaptic cell membrane, postsynaptic density, synapse |
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