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Human PLA2G7/PAF-AH/Lp-PLA2 Rabbit pAb (A24499)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - Human PLA2G7/PAF-AH/Lp-PLA2 Rabbit pAb (A24499)

Western blot analysis of Human plasma, using Human PLA2G7/PAF-AH/Lp-PLA2 Rabbit pAb (A24499) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameHuman PLA2G7/PAF-AH/Lp-PLA2 Rabbit pAb
Catalog No.A24499
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 22-441 of human PLA2G7/PAF-AH/Lp-PLA2 (NP_005075.3).
SequenceFDWQYINPVAHMKSSAWVNKIQVLMAAASFGQTKIPRGNGPYSVGCTDLMFDHTNKGTFLRLYYPSQDNDRLDTLWIPNKEYFWGLSKFLGTHWLMGNILRLLFGSMTTPANWNSPLRPGEKYPLVVFSHGLGAFRTLYSAIGIDLASHGFIVAAVEHRDRSASATYYFKDQSAAEIGDKSWLYLRTLKQEEETHIRNEQVRQRAKECSQALSLILDIDHGKPVKNALDLKFDMEQLKDSIDREKIAVIGHSFGGATVIQTLSEDQRFRCGIALDAWMFPLGDEVYSRIPQPLFFINSEYFQYPANIIKMKKCYSPDKERKMITIRGSVHQNFADFTFATGKIIGHMLKLKGDIDSNVAIDLSNKASLAFLQKHLGLHKDFDQWDCLIEGDDENLIPGTNINTTNQHIMLQNSSGIEKYN
Gene ID
Swiss Prot
SynonymsPLA2G7; LDL-PLA2; LP-PLA2; PAFAD; PAFAH; phospholipase A2 group VII
Calculated MW50kDa
Observed MW60kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHuman plasma
Cellular locationSecreted, extracellular space

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Human PLA2G7/PAF-AH/Lp-PLA2 Rabbit pAb (A24499)}

Western blot - Human PLA2G7/PAF-AH/Lp-PLA2 Rabbit pAb (A24499)

Western blot analysis of Human plasma, using Human PLA2G7/PAF-AH/Lp-PLA2 Rabbit pAb (A24499) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

재조합 단백질 (1)

Secondary Antibodies (26)