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Hydroxyl-Histone H2A-Y39 Rabbit mAb (A4827)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

Western blot analysis of various lysates using Hydroxyl-Histone H2A-Y39 Rabbit mAb (A4827) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Hydroxyl-Histone H2A-Y39 Rabbit mAb (A4827)

Immunohistochemistry analysis of Hydroxyl-Histone H2A-Y39 in paraffin-embedded human small intestine tissue using Hydroxyl-Histone H2A-Y39 Rabbit mAb (A4827) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of Hydroxyl-Histone H2A-Y39 in paraffin-embedded mouse colon tissue using Hydroxyl-Histone H2A-Y39 Rabbit mAb (A4827) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of Hydroxyl-Histone H2A-Y39 in paraffin-embedded rat brain tissue using Hydroxyl-Histone H2A-Y39 Rabbit mAb (A4827) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of Hydroxyl-Histone H2A-Y39 in paraffin-embedded rat colon tissue using Hydroxyl-Histone H2A-Y39 Rabbit mAb (A4827) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of Hydroxyl-Histone H2A-Y39 in paraffin-embedded human esophagus tissue using Hydroxyl-Histone H2A-Y39 Rabbit mAb (A4827) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product name	Hydroxyl-Histone H2A-Y39 Rabbit mAb
Catalog No.	A4827
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0253

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen	A synthetic hydroxylated peptide around Y39 of human Histone H2A (P04908).
Sequence	KGNYS
Gene ID
Swiss Prot
Synonyms	H2A.1; H2A.2; H2A/a; H2AC4; H2AFA; HIST1H2AE; Hydroxyl-Histone H2A-Y39
Calculated MW	14kDa
Observed MW	17kDa

Applications

Reactivity	Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	HeLa, 293T, Mouse thymus, Rat liver
Cellular location	Chromosome, Nucleus