Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Western blot analysis of lysates from C6 cells, using Ubiquityl-Histone H2A-K119 Rabbit pAb (A17952) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
| Product name | Ubiquityl-Histone H2A-K119 Rabbit pAb |
|---|---|
| Catalog No. | A17952 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic ubiquitinated peptide around K119 of human HIST1H2AB (NP_734466.1). |
|---|---|
| Sequence | LPKKT |
| Gene ID | |
| Swiss Prot | |
| Synonyms | HIST1H2AB; Ubiquityl-Histone H2A-K119 |
| Calculated MW | 14kDa |
| Observed MW | 23kDa |
| Reactivity | Human, Mouse, Rat, Other (Wide Range Predicted) |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | C6 |
| Cellular location | extracellular exosome, nucleus. |
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