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IAPP Rabbit pAb (A2816)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - IAPP Rabbit pAb (A2816)

Western blot analysis of lysates from 293F-IAPP cells, using IAPP Rabbit pAb (A2816) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - IAPP Rabbit pAb (A2816)

Immunohistochemistry analysis of paraffin-embedded Rat pancreas using IAPP Rabbit pAb (A2816) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - IAPP Rabbit pAb (A2816)

Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using IAPP Rabbit pAb (A2816) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - IAPP Rabbit pAb (A2816)

Immunofluorescence analysis of paraffin-embedded human pancreas using IAPP Rabbit pAb (A2816) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - IAPP Rabbit pAb (A2816)

Immunofluorescence analysis of paraffin-embedded mouse pancreas using IAPP Rabbit pAb (A2816) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameIAPP Rabbit pAb
Catalog No.A2816
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the calcitonin family of peptide hormones. This hormone is released from pancreatic beta cells following food intake to regulate blood glucose levels and act as a satiation signal. Human patients with type 1 and advanced type 2 diabetes exhibit reduced levels of the encoded hormone in blood and pancreas. This protein also exhibits a bactericidal, antimicrobial activity.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-89 of human IAPP (NP_000406.1).
SequenceMGILKLQVFLIVLSVALNHLKATPIESHQVEKRKCNTATCATQRLANFLVHSSNNFGAILSSTNVGSNTYGKRNAVEVLKREPLNYLPL
Gene ID
Swiss Prot
SynonymsDAP; IAP; IAPP
Calculated MW10kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293F, 293F-IAPP
Cellular locationSecreted
Customer validation

WB(Homo sapiens)

IF(Homo sapiens)

IP(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - IAPP Rabbit pAb (A2816)}

Western blot - IAPP Rabbit pAb (A2816)

Western blot analysis of lysates from 293F-IAPP cells, using IAPP Rabbit pAb (A2816) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - IAPP Rabbit pAb (A2816)}

Immunohistochemistry - IAPP Rabbit pAb (A2816)

Immunohistochemistry analysis of paraffin-embedded Rat pancreas using IAPP Rabbit pAb (A2816) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - IAPP Rabbit pAb (A2816)}

Immunohistochemistry - IAPP Rabbit pAb (A2816)

Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using IAPP Rabbit pAb (A2816) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - IAPP Rabbit pAb (A2816)}

Immunofluorescence - IAPP Rabbit pAb (A2816)

Immunofluorescence analysis of paraffin-embedded human pancreas using IAPP Rabbit pAb (A2816) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - IAPP Rabbit pAb (A2816)}

Immunofluorescence - IAPP Rabbit pAb (A2816)

Immunofluorescence analysis of paraffin-embedded mouse pancreas using IAPP Rabbit pAb (A2816) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)