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ICOS/CD278 Rabbit pAb (A1811)

Review (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ICOS/CD278 Rabbit pAb (A1811)

Western blot analysis of various lysates using ICOS/CD278 Rabbit pAb (A1811) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - ICOS/CD278 Rabbit pAb (A1811)

Immunofluorescence analysis of HL-60 cells using ICOS/CD278 Rabbit pAb (A1811) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ICOS/CD278 Rabbit pAb (A1811)

Immunofluorescence analysis of Jurkat cells using ICOS/CD278 Rabbit pAb (A1811) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for ICOS/CD278 Rabbit pAb(A1811)

Overview

Product nameICOS/CD278 Rabbit pAb
Catalog No.A1811
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 21-140 of human ICOS/CD278 (NP_036224.1).
SequenceEINGSANYEMFIFHNGGVQILCKYPDIVQQFKMQLLKGGQILCDLTKTKGSGNTVSIKSLKFCHSQLSNNSVSFFLYNLDHSHANYYFCNLSIFDPPPFKVTLTGGYLHIYESQLCCQLK
Gene ID
Swiss Prot
SynonymsAILIM; CD278; CVID1; ICOS/CD278
Calculated MW23kDa
Observed MW30-40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesJurkat, Mouse thymus, Mouse spleen, Rat thymus
Cellular locationCell membrane, Secreted, Single-pass type I membrane protein.
Customer validation

(Human,Mouse)

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - ICOS/CD278 Rabbit pAb (A1811)}

Western blot - ICOS/CD278 Rabbit pAb (A1811)

Western blot analysis of various lysates using ICOS/CD278 Rabbit pAb (A1811) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - ICOS/CD278 Rabbit pAb (A1811)}

Immunofluorescence - ICOS/CD278 Rabbit pAb (A1811)

Immunofluorescence analysis of HL-60 cells using ICOS/CD278 Rabbit pAb (A1811) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ICOS/CD278 Rabbit pAb (A1811)}

Immunofluorescence - ICOS/CD278 Rabbit pAb (A1811)

Immunofluorescence analysis of Jurkat cells using ICOS/CD278 Rabbit pAb (A1811) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

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Secondary Antibodies (26)