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IFI16 Rabbit pAb (A14002)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - IFI16 Rabbit pAb (A14002)

Western blot analysis of various lysates, using IFI16 Rabbit pAb (A14002) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

ABclonal:Immunohistochemistry - IFI16 Rabbit pAb (A14002)

Immunohistochemistry analysis of paraffin-embedded rat liver using IFI16 Rabbit pAb (A14002) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - IFI16 Rabbit pAb (A14002)

Immunohistochemistry analysis of paraffin-embedded human kidney cancer using IFI16 Rabbit pAb (A14002) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - IFI16 Rabbit pAb (A14002)

Immunohistochemistry analysis of paraffin-embedded mouse lung using IFI16 Rabbit pAb (A14002) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameIFI16 Rabbit pAb
Catalog No.A14002
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human IFI16 (NP_001351796.1).
SequenceMGKKYKNIVLLKGLEVINDYHFRMVKSLLSNDLKLNLKMREEYDKIQIADLMEEKFRGDAGLGKLIKIFEDIPTLEDLAETLKKEKLKVKGPALSRKRKKEVDATSPAPSTSSTVKTEGAEATPGAQKRKKSTKEKAGPKGSKVSEEQTQPPSPAGAGMSTAMGRSPSPKTSLSAPPNSSSTENPKTVAKCQVTPRRNVLQKRPVIVKVLSTTKPFEYETPEMEKKIMFHATVATQTQFFHVKVLNTSLKEKFNGKKIIIISDYLEYDSLLEVNEESTVSEAGPNQTFEVPNKIINRAKE
Gene ID
Swiss Prot
SynonymsPYHIN2; IFNGIP1; IFI16
Calculated MW88kDa
Observed MW85-98kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesU-87MG, Daudi
Cellular locationCytoplasm, Nucleus
Customer validation

IHC(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - IFI16 Rabbit pAb (A14002)}

Western blot - IFI16 Rabbit pAb (A14002)

Western blot analysis of various lysates, using IFI16 Rabbit pAb (A14002) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.
ABclonal:Immunohistochemistry - IFI16 Rabbit pAb (A14002)}

Immunohistochemistry - IFI16 Rabbit pAb (A14002)

Immunohistochemistry analysis of paraffin-embedded rat liver using IFI16 Rabbit pAb (A14002) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - IFI16 Rabbit pAb (A14002)}

Immunohistochemistry - IFI16 Rabbit pAb (A14002)

Immunohistochemistry analysis of paraffin-embedded human kidney cancer using IFI16 Rabbit pAb (A14002) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - IFI16 Rabbit pAb (A14002)}

Immunohistochemistry - IFI16 Rabbit pAb (A14002)

Immunohistochemistry analysis of paraffin-embedded mouse lung using IFI16 Rabbit pAb (A14002) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A14002? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)