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IFIT3 Rabbit pAb (A3924)

Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - IFIT3 Rabbit pAb (A3924)

Western blot analysis of various lysates using IFIT3 Rabbit pAb (A3924) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

ABclonal:Immunohistochemistry - IFIT3 Rabbit pAb (A3924)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using IFIT3 Rabbit pAb (A3924) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - IFIT3 Rabbit pAb (A3924)

Immunohistochemistry analysis of paraffin-embedded human liver using IFIT3 Rabbit pAb (A3924) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameIFIT3 Rabbit pAb
Catalog No.A3924
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable RNA binding activity and identical protein binding activity. Acts upstream of or within cellular response to interferon-beta; response to bacterium; and response to stilbenoid. Predicted to be located in mitochondrion. Predicted to be active in cytosol. Is expressed in alimentary system; eye; face; and nervous system. Orthologous to human IFIT3 (interferon induced protein with tetratricopeptide repeats 3).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 128-331 of mouse IFIT3 (NP_034631.1).
SequenceANPYSMECPELECEEGWTRLKCGRNERAKMCFEKALEEKPKDPECSSGMAIAMFRLEEKPEKQFSVDALKQAMELNPQNQYLKVLLALKLLRMGEEAEGERLIKDALGKAPNQTDVLQKAAQFYKKKGNLDRAIELLGKALRSTVNNSPLYSLVMCRYREILEQLQNKGDADSSERRQRMAELRRLTMEFMQKTLQRRRSPLNS
Gene ID
Swiss Prot
SynonymsP49; Ifi49; IFIT3
Calculated MW56kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:100 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse kidney, Rat liver
Cellular locationCytoplasm, Mitochondrion
Customer validation

WB(Mus musculus)

IHC(Rattus norvegicus)

Documents

Certificate of Compliance

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    ABclonal:Western blot - IFIT3 Rabbit pAb (A3924)}

    Western blot - IFIT3 Rabbit pAb (A3924)

    Western blot analysis of various lysates using IFIT3 Rabbit pAb (A3924) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 60s.
    ABclonal:Immunohistochemistry - IFIT3 Rabbit pAb (A3924)}

    Immunohistochemistry - IFIT3 Rabbit pAb (A3924)

    Immunohistochemistry analysis of paraffin-embedded human lung cancer using IFIT3 Rabbit pAb (A3924) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - IFIT3 Rabbit pAb (A3924)}

    Immunohistochemistry - IFIT3 Rabbit pAb (A3924)

    Immunohistochemistry analysis of paraffin-embedded human liver using IFIT3 Rabbit pAb (A3924) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A3924? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    Secondary Antibodies (26)