제품 > 항체 > 다중클론항체(pAb)

IFT88 Rabbit pAb (A4204)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of extracts of CEM cells, using IFT88 antibody (A4204).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Overview

Product name	IFT88 Rabbit pAb
Catalog No.	A4204
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the tetratrico peptide repeat (TPR) family. The encoded protein is involved in cilium biogenesis. Mutations of a similar gene in mouse can cause polycystic kidney disease. Several transcript variants encoding distinct isoforms have been identified for this gene.

Immunogen information

Immunogen	A synthetic Peptide of human IFT88
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	DAF19; TG737; TTC10; hTg737; D13S1056E
Calculated MW	93kDa
Observed MW	109kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:20 - 1:50
Storage buffer	Store at 4℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	CEM
Cellular location	Cytoplasm, centriole, centrosome, cilium basal body, cytoskeleton, microtubule organizing center
Customer validation	WB(Mus musculus) IHC(Mus musculus)