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IGFBP1 Rabbit mAb (A11672)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - IGFBP1 Rabbit mAb (A11672)

Western blot analysis of extracts of HepG2 cells, using IGFBP1 Rabbit mAb (A11672) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - IGFBP1 Rabbit mAb (A11672)

Western blot analysis of extracts of Mouse liver, using IGFBP1 antibody (A11672) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

Overview

Product nameIGFBP1 Rabbit mAb
Catalog No.A11672
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0671
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP N-terminal domain and a thyroglobulin type-I domain. The encoded protein, mainly expressed in the liver, circulates in the plasma and binds both insulin-like growth factors (IGFs) I and II, prolonging their half-lives and altering their interaction with cell surface receptors. This protein is important in cell migration and metabolism. Low levels of this protein may be associated with impaired glucose tolerance, vascular disease and hypertension in human patients.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 160-259 of human IGFBP1 (P08833).
SequenceGSKALHVTNIKKWKEPCRIELYRVVESLAKAQETSGEEISKFYLPNCNKNGFYHSRQCETSMDGEAGLCWCVYPWNGKRIPGSPEIRGDPNCQIYFNVQN
Gene ID
Swiss Prot
SynonymsAFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
Calculated MW28kDa
Observed MW30kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHep G2, Mouse liver
Cellular locationSecreted
Customer validation

IP(Mus musculus)

WB(Homo sapiens,Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - IGFBP1 Rabbit mAb (A11672)}

Western blot - IGFBP1 Rabbit mAb (A11672)

Western blot analysis of extracts of HepG2 cells, using IGFBP1 Rabbit mAb (A11672) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - IGFBP1 Rabbit mAb (A11672)}

Western blot - IGFBP1 Rabbit mAb (A11672)

Western blot analysis of extracts of Mouse liver, using IGFBP1 antibody (A11672) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

재조합 단백질 (1)

Secondary Antibodies (26)