제품 > 항체 > 다중클론항체(pAb)

IL11 Rabbit pAb (A1902)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using IL11 Rabbit pAb (A1902) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - IL11 Rabbit pAb (A1902)

Immunofluorescence analysis of L929 cells using IL11 Rabbit pAb (A1902) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	IL11 Rabbit pAb
Catalog No.	A1902
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a member of the gp130 family of cytokines. These cytokines drive the assembly of multisubunit receptor complexes, all of which contain at least one molecule of the transmembrane signaling receptor IL6ST (gp130). This cytokine is shown to stimulate the T-cell-dependent development of immunoglobulin-producing B cells. It is also found to support the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 22-199 of human IL11 (NP_000632.1).
Sequence	PGPPPGPPRVSPDPRAELDSTVLLTRSLLADTRQLAAQLRDKFPADGDHNLDSLPTLAMSAGALGALQLPGVLTRLRADLLSYLRHVQWLRRAGGSSLKTLEPELGTLQARLDRLLRRLQLLMSRLALPQPPPDPPAPPLAPPSSAWGGIRAAHAILGGLHLTLDWAVRGLLLLKTRL
Gene ID
Swiss Prot
Synonyms	AGIF; IL-11; IL11
Calculated MW	21kDa
Observed MW	25kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HuH-7, Mouse testis, Rat testis
Cellular location	Secreted.
Customer validation	WB(Mus musculus, Homo sapiens, Mus musculus, Oryctolagus cuniculus) IHC(Mus musculus, Homo sapiens) IF(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

IL11 Rabbit pAb images

Western blot - IL11 Rabbit pAb (A1902)

Immunofluorescence - IL11 Rabbit pAb (A1902)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for IL11 Rabbit pAb (A1902)

Product:

IL11 Rabbit pAb

Journal:

Int J Mol Med

Application:

WB, IHC

IF:

5.7

Species:

Mus musculus

PMID:

34414450

Title:

Omega‑3 polyunsaturated fatty acids inhibit IL‑11/STAT3 signaling in hepatocytes during acetaminophen hepatotoxicity

References for IL11 Rabbit pAb (A1902)

Product:

IL11 Rabbit pAb

Journal:

FASEB J

Application:

WB, IHC

IF:

4.8

Species:

Homo sapiens

PMID:

34042220

Title:

miR‐10396b‐3p inhibits mechanical stress‐induced ligamentum flavum hypertrophy by targeting IL‐11

References for IL11 Rabbit pAb (A1902)

Product:

IL11 Rabbit pAb

Journal:

Cell proliferation

Application:

IF:

8.5

Species:

Homo sapiens

PMID:

36515067

Title:

Comprehensive analysis of immune cell infiltration and role of MSR1 expression in aneurysmal subarachnoid haemorrhage

References for IL11 Rabbit pAb (A1902)

Product:

IL11 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

5.6

Species:

Mus musculus

PMID:

36292942

Title:

Oxygen-Glucose Deprivation Promoted Fibroblast Senescence and Collagen Expression via IL11 - PMC

References for IL11 Rabbit pAb (A1902)

Product:

IL11 Rabbit pAb

Journal:

American journal of cancer research

Application:

IF:

3.6

Species:

Homo sapiens

PMID:

37693165

Title:

Identification of ARAP3 as a regulator of tumor progression, macrophage infiltration and osteoclast differentiation in a tumor microenvironment-related prognostic model of Ewing sarcoma - PMC

References for IL11 Rabbit pAb (A1902)

Product:

IL11 Rabbit pAb

Journal:

Life sciences

Application:

IF:

5.2

Species:

Mus musculus

PMID:

37495078

Title:

The effect of hepatic stellate cell derived-IL-11 on hepatocyte injury in hepatic fibrosis

References for IL11 Rabbit pAb (A1902)

Product:

IL11 Rabbit pAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IF:

6.9

Species:

Homo sapiens, Mus musculus

PMID:

37776637

Title:

Biomimetic nanoparticles loaded lutein functionalized by macrophage membrane for targeted amelioration pressure overload-induced cardiac fibrosis

References for IL11 Rabbit pAb (A1902)

Product:

IL11 Rabbit pAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

IF:

15.1

Species:

Homo sapiens

PMID:

37387572

Title:

Autophagosomes Defeat Ferroptosis by Decreasing Generation and Increasing Discharge of Free Fe2+ in Skin Repair Cells to Accelerate Diabetic Wound Healing

References for IL11 Rabbit pAb (A1902)

Product:

IL11 Rabbit pAb

Journal:

International journal of pharmaceutics

Application:

IF:

5.3

Species:

Oryctolagus cuniculus

PMID:

38701907

Title:

Construction of cryomicroneedles loaded with milk-derived exosomes encapsulated TNF- siRNA and efficacy of percutaneous acupoint administration in rheumatoid

References for IL11 Rabbit pAb (A1902)

Product:

IL11 Rabbit pAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

IF:

15.1

Species:

Mus musculus

PMID:

38696655