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IL1RA Rabbit pAb (A2088)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - IL1RA Rabbit pAb (A2088)

Western blot analysis of extracts of A-431 cells, using IL1RA antibody (A2088) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - IL1RA Rabbit pAb (A2088)

Immunofluorescence analysis of human skin cancer using IL1RA Rabbit pAb (A2088) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - IL1RA Rabbit pAb (A2088)

Immunofluorescence analysis of A431 cells using IL1RA Rabbit pAb (A2088) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - IL1RA Rabbit pAb (A2088)

Immunofluorescence analysis of HepG2 cells using IL1RA Rabbit pAb (A2088) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameIL1RA Rabbit pAb
Catalog No.A2088
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses, particularly in the acute phase of infection and inflammation. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 24-177 of human IL1RA (NP_776214.1).
SequenceICRPSGRKSSKMQAFRIWDVNQKTFYLRNNQLVAGYLQGPNVNLEEKIDVVPIEPHALFLGIHGGKMCLSCVKSGDETRLQLEAVNITDLSENRKQDKRFAFIRSDSGPTTSFESAACPGWFLCTAMEADQPVSLTNMPDEGVMVTKFYFQEDE
Gene ID
Swiss Prot
SynonymsDIRA; IRAP; IL1F3; IL1RA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA
Calculated MW20kDa
Observed MW18kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesA-431
Cellular locationCytoplasm, Secreted
Customer validation

WB(Homo sapiens, Mus musculus)

IP(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - IL1RA Rabbit pAb (A2088)}

Western blot - IL1RA Rabbit pAb (A2088)

Western blot analysis of extracts of A-431 cells, using IL1RA antibody (A2088) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - IL1RA Rabbit pAb (A2088)}

Immunofluorescence - IL1RA Rabbit pAb (A2088)

Immunofluorescence analysis of human skin cancer using IL1RA Rabbit pAb (A2088) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - IL1RA Rabbit pAb (A2088)}

Immunofluorescence - IL1RA Rabbit pAb (A2088)

Immunofluorescence analysis of A431 cells using IL1RA Rabbit pAb (A2088) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - IL1RA Rabbit pAb (A2088)}

Immunofluorescence - IL1RA Rabbit pAb (A2088)

Immunofluorescence analysis of HepG2 cells using IL1RA Rabbit pAb (A2088) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A2088? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

재조합 단백질 (1)

ELISA 키트 (1)

Secondary Antibodies (26)