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IL21 Rabbit pAb (A7235)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - IL21 Rabbit pAb (A7235)

Western blot analysis of various lysates using (A7235) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Western blot - IL21 Rabbit pAb (A7235)

Western blot analysis of lysates from Rat spleen, using (A7235) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - IL21 Rabbit pAb (A7235)

Immunohistochemistry analysis of paraffin-embedded Human colon using IL21 Rabbit pAb (A7235) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - IL21 Rabbit pAb (A7235)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using IL21 Rabbit pAb (A7235) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - IL21 Rabbit pAb (A7235)

Immunohistochemistry analysis of paraffin-embedded Rat lung using IL21 Rabbit pAb (A7235) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - IL21 Rabbit pAb (A7235)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using IL21 Rabbit pAb (A7235) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - IL21 Rabbit pAb (A7235)

Immunofluorescence analysis of HeLa cells using IL21 Rabbit pAb (A7235) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameIL21 Rabbit pAb
Catalog No.A7235
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the common-gamma chain family of cytokines with immunoregulatory activity. The encoded protein plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. Dysregulation of this gene plays a role in multiple immune-mediated diseases including lupus, psoriasis and chronic inflammatory diseases. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 25-162 of human IL21 (NP_068575.1).
SequenceHKSSSQGQDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANTGNNERIINVSIKKLKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIHQHLSSRTHGSEDS
Gene ID
Swiss Prot
SynonymsZa11; IL-21; CVID11; IL21
Calculated MW19kDa
Observed MW19kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesRaji, Mouse thymus, Rat spleen
Cellular locationSecreted.
Customer validation

WB(Mus musculus, Rattus norvegicus)

Documents

Certificate of Compliance

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ABclonal:Western blot - IL21 Rabbit pAb (A7235)}

Western blot - IL21 Rabbit pAb (A7235)

Western blot analysis of various lysates using (A7235) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Western blot - IL21 Rabbit pAb (A7235)}

Western blot - IL21 Rabbit pAb (A7235)

Western blot analysis of lysates from Rat spleen, using (A7235) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - IL21 Rabbit pAb (A7235)}

Immunohistochemistry - IL21 Rabbit pAb (A7235)

Immunohistochemistry analysis of paraffin-embedded Human colon using IL21 Rabbit pAb (A7235) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - IL21 Rabbit pAb (A7235)}

Immunohistochemistry - IL21 Rabbit pAb (A7235)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using IL21 Rabbit pAb (A7235) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - IL21 Rabbit pAb (A7235)}

Immunohistochemistry - IL21 Rabbit pAb (A7235)

Immunohistochemistry analysis of paraffin-embedded Rat lung using IL21 Rabbit pAb (A7235) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - IL21 Rabbit pAb (A7235)}

Immunohistochemistry - IL21 Rabbit pAb (A7235)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using IL21 Rabbit pAb (A7235) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - IL21 Rabbit pAb (A7235)}

Immunofluorescence - IL21 Rabbit pAb (A7235)

Immunofluorescence analysis of HeLa cells using IL21 Rabbit pAb (A7235) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A7235? Please let us know so that we can cite the reference in this datasheet.

ELISA 키트 (1)

재조합 단백질 (1)

항체 (3)

Secondary Antibodies (26)