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IL23R Rabbit mAb (A22561)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - IL23R Rabbit mAb (A22561)

Western blot analysis of various lysates using IL23R Rabbit mAb (A22561) at 1:20000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): HeLa
Exposure time: 180s.

ABclonal:Immunohistochemistry - IL23R Rabbit mAb (A22561)

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using IL23R Rabbit mAb (A22561) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - IL23R Rabbit mAb (A22561)

Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using IL23R Rabbit mAb (A22561) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - IL23R Rabbit mAb (A22561)

Immunohistochemistry analysis of paraffin-embedded Rat intestine tissue using IL23R Rabbit mAb (A22561) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product nameIL23R Rabbit mAb
Catalog No.A22561
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC58334
The protein encoded by this gene is a subunit of the receptor for IL23A/IL23. This protein pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. This protein associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand-dependent manner.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 510-629 of human IL23R (NP_653302.2).
SequenceKPPVDSLDSGNNPRLQKHPNFAFSVSSVNSLSNTIFLGELSLILNQGECSSPDIQNSVEEETTMLLENDSPSETIPEQTLLPDEFVSCLGIVNEELPSINTYFPQNILESHFNRISLLEK
Gene ID
Swiss Prot
SynonymsPSORS7; IL23R
Calculated MW72kDa
Observed MW72kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:20000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesJurkat, Raji, RAW264.7, Mouse thymus
Cellular locationCell membrane, Single-pass type I membrane protein.

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ABclonal:Western blot - IL23R Rabbit mAb (A22561)}

Western blot - IL23R Rabbit mAb (A22561)

Western blot analysis of various lysates using IL23R Rabbit mAb (A22561) at 1:20000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): HeLa
Exposure time: 180s.
ABclonal:Immunohistochemistry - IL23R Rabbit mAb (A22561)}

Immunohistochemistry - IL23R Rabbit mAb (A22561)

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using IL23R Rabbit mAb (A22561) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - IL23R Rabbit mAb (A22561)}

Immunohistochemistry - IL23R Rabbit mAb (A22561)

Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using IL23R Rabbit mAb (A22561) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - IL23R Rabbit mAb (A22561)}

Immunohistochemistry - IL23R Rabbit mAb (A22561)

Immunohistochemistry analysis of paraffin-embedded Rat intestine tissue using IL23R Rabbit mAb (A22561) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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