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ILF2 Rabbit mAb (A9530)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ILF2 Rabbit mAb (A9530)

Western blot analysis of various lysates using ILF2 Rabbit mAb (A9530) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - ILF2 Rabbit mAb (A9530)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using ILF2 Rabbit mAb (A9530) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product nameILF2 Rabbit mAb
Catalog No.A9530
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1621
The protein encoded by this gene is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm, to repair DNA breaks by nonhomologous end joining, and to negatively regulate the microRNA processing pathway. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-300 of human ILF2 (Q12905).
SequenceSALAAIRHARWFEENASQSTVKVLIRLLKDLRIRFPGFEPLTPWILDLLGHYAVMNNPTRQPLALNVAYRRCLQILAAGLFLPGSVGITDPCESGNFRVHT
Gene ID
Swiss Prot
SynonymsNF45; PRO3063; ILF2
Calculated MW43kDa
Observed MW43kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, HepG2, Raji, Mouse thymus, Rat testis
Cellular locationextracellular region, nucleolus, nucleoplasm, nucleus

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ABclonal:Western blot - ILF2 Rabbit mAb (A9530)}

Western blot - ILF2 Rabbit mAb (A9530)

Western blot analysis of various lysates using ILF2 Rabbit mAb (A9530) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - ILF2 Rabbit mAb (A9530)}

Immunohistochemistry - ILF2 Rabbit mAb (A9530)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using ILF2 Rabbit mAb (A9530) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

Secondary Antibodies (26)