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ILF3 Rabbit mAb (A8186)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ILF3 Rabbit mAb (A8186)

Western blot analysis of various lysates using ILF3 Rabbit mAb (A8186) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded human colon carcinoma tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded human colon tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded human cervix cancer tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded human thyroid cancer tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded mouse lung tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded mouse testis tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded rat colon tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded mouse colon tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded rat liver tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded human liver tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded mouse brain tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameILF3 Rabbit mAb
Catalog No.A8186
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1438
This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human ILF3 (Q12906).
SequenceMRPMRIFVNDDRHVMAKHSSVYPTQEELEAVQNMVSHTERALKAVSDWIDEQEKGSSEQAESDNMDVPPEDDSKEGAGEQKTEHMTRTLRGVMRVGLVAK
Gene ID
Swiss Prot
SynonymsCBTF; DRBF; MMP4; MPP4; NF90; NFAR; NF110; NF90a; NF90b; NF90c; NFAR2; TCP80; DRBP76; NF110b; NFAR-1; NFAR-2; NFAR90; TCP110; MPP4110; NF90ctv; NFAR110; MPHOSPH4; NF-AT-90; ILF3
Calculated MW95kDa
Observed MW90kDa/100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, Raji, C6, SH-SY5Y, Mouse testis
Cellular locationCytoplasm, Nucleus, Nucleolus
Customer validation

IHC(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - ILF3 Rabbit mAb (A8186)}

Western blot - ILF3 Rabbit mAb (A8186)

Western blot analysis of various lysates using ILF3 Rabbit mAb (A8186) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)}

Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded human colon carcinoma tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)}

Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded human colon tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)}

Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded human cervix cancer tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)}

Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded human thyroid cancer tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)}

Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded mouse lung tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)}

Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded mouse testis tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)}

Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded rat colon tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)}

Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded mouse colon tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)}

Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded rat liver tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)}

Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded human liver tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ILF3 Rabbit mAb (A8186)}

Immunohistochemistry - ILF3 Rabbit mAb (A8186)

Immunohistochemistry analysis of ILF3 in paraffin-embedded mouse brain tissue using ILF3 Rabbit mAb (A8186) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)