제품 > 항체 > 다중클론항체(pAb)

INPP5K Rabbit pAb (A7807)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - INPP5K Rabbit pAb (A7807)

Western blot analysis of lysates from 293T cells, using INPP5K Rabbit pAb (A7807) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - INPP5K Rabbit pAb (A7807)

Western blot analysis of lysates from Mouse skeletal muscle, using INPP5K Rabbit pAb (A7807) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - INPP5K Rabbit pAb (A7807)

Immunofluorescence analysis of A-549 cells using INPP5K Rabbit pAb (A7807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - INPP5K Rabbit pAb (A7807)

Immunofluorescence analysis of HeLa cells using INPP5K Rabbit pAb (A7807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - INPP5K Rabbit pAb (A7807)

Immunofluorescence analysis of NIH/3T3 cells using INPP5K Rabbit pAb (A7807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - INPP5K Rabbit pAb (A7807)

Immunofluorescence analysis of PC-12 cells using INPP5K Rabbit pAb (A7807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameINPP5K Rabbit pAb
Catalog No.A7807
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein with 5-phosphatase activity toward polyphosphate inositol. The protein localizes to the cytosol in regions lacking actin stress fibers. It is thought that this protein may negatively regulate the actin cytoskeleton. Alternatively spliced transcript variants encoding different isoforms have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 149-448 of human INPP5K (NP_057616.2).
SequenceHLPPHISNNYQRLEHFDRILEMQNCEGRDIPNILDHDLIIWFGDMNFRIEDFGLHFVRESIKNRCYGGLWEKDQLSIAKKHDPLLREFQEGRLLFPPTYKFDRNSNDYDTSEKKRKPAWTDRILWRLKRQPCAGPDTPIPPASHFSLSLRGYSSHMTYGISDHKPVSGTFDLELKPLVSAPLIVLMPEDLWTVENDMMVSYSSTSDFPSSPWDWIGLYKVGLRDVNDYVSYAWVGDSKVSCSDNLNQVYIDISNIPTTEDEFLLCYYSNSLRSVVGISRPFQIPPGSLREDPLGEAQPQI
Gene ID
Swiss Prot
SynonymsPPS; SKIP; MDCCAID; INPP5K
Calculated MW51kDa
Observed MW51kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T, Mouse skeletal muscle
Cellular locationEndoplasmic reticulum

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - INPP5K Rabbit pAb (A7807)}

    Western blot - INPP5K Rabbit pAb (A7807)

    Western blot analysis of lysates from 293T cells, using INPP5K Rabbit pAb (A7807) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - INPP5K Rabbit pAb (A7807)}

    Western blot - INPP5K Rabbit pAb (A7807)

    Western blot analysis of lysates from Mouse skeletal muscle, using INPP5K Rabbit pAb (A7807) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Immunofluorescence - INPP5K Rabbit pAb (A7807)}

    Immunofluorescence - INPP5K Rabbit pAb (A7807)

    Immunofluorescence analysis of A-549 cells using INPP5K Rabbit pAb (A7807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - INPP5K Rabbit pAb (A7807)}

    Immunofluorescence - INPP5K Rabbit pAb (A7807)

    Immunofluorescence analysis of HeLa cells using INPP5K Rabbit pAb (A7807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - INPP5K Rabbit pAb (A7807)}

    Immunofluorescence - INPP5K Rabbit pAb (A7807)

    Immunofluorescence analysis of NIH/3T3 cells using INPP5K Rabbit pAb (A7807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - INPP5K Rabbit pAb (A7807)}

    Immunofluorescence - INPP5K Rabbit pAb (A7807)

    Immunofluorescence analysis of PC-12 cells using INPP5K Rabbit pAb (A7807) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.