제품 > 항체 > 다중클론항체(pAb)

INSM1 Rabbit pAb (A20424)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - INSM1 Rabbit pAb (A20424)

Western blot analysis of various lysates using INSM1 Rabbit pAb (A20424) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - INSM1 Rabbit pAb (A20424)

Immunohistochemistry analysis of paraffin-embedded human small cell lung cancer using INSM1 Rabbit pAb (A20424) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameINSM1 Rabbit pAb
Catalog No.A20424
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Insulinoma-associated 1 (INSM1) gene is intronless and encodes a protein containing both a zinc finger DNA-binding domain and a putative prohormone domain. This gene is a sensitive marker for neuroendocrine differentiation of human lung tumors.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 360-469 of human INSM1 (NP_002187.1).
SequenceSGSEDGLYECHHCAKKFRRQAYLRKHLLAHHQALQAKGAPLAPPAEDLLALYPGPDEKAPQEAAGDGEGAGVLGLSASAECHLCPVCGESFASKGAQERHLRLLHAAQVF
Gene ID
Swiss Prot
SynonymsIA1; IA-1; INSM1
Calculated MW53kDa
Observed MW53kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesSH-SY5Y, Mouse pancreas
Cellular locationnucleoplasm, nucleus

Documents

Certificate of Compliance

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    ABclonal:Western blot - INSM1 Rabbit pAb (A20424)}

    Western blot - INSM1 Rabbit pAb (A20424)

    Western blot analysis of various lysates using INSM1 Rabbit pAb (A20424) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - INSM1 Rabbit pAb (A20424)}

    Immunohistochemistry - INSM1 Rabbit pAb (A20424)

    Immunohistochemistry analysis of paraffin-embedded human small cell lung cancer using INSM1 Rabbit pAb (A20424) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.