제품 > 항체 > 다중클론항체(pAb)

IPA1 Rabbit pAb (A17556)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - IPA1 Rabbit pAb (A17556)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using IPA1 Rabbit pAb (A17556) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 600s.

Overview

Product nameIPA1 Rabbit pAb
Catalog No.A17556
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Trans-acting factor that binds specifically to the consensus nucleotide sequence 5'-TNCGTACAA-3' (By similarity). May be involved in panicle development.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 209-417 of Oryza sativa IPA1. (Q7EXZ2).
SequencePRVPSSVRNAWPAIQPGDRISGGIQWHRNVAPHGHSSAVAGYGANTYSGQGSSSSGPPVFAGPNLPPGGCLAGVGAATDSSCALSLLSTQPWDTTTHSAAASHNQAAAMSTTTSFDGNPVAPSAMAGSYMAPSPWTGSRGHEGGGRSVAHQLPHEVSLDEVHPGPSHHAHFSGELELALQGNGPAPAPRIDPGSGSTFDQTSNTMDWSL
Gene ID
Swiss Prot
SynonymsWFP; IPA1; SPL14; OsSPL14
Calculated MW42kDa
Observed MW52kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesstems (before flower), leaves sheath (before flower), flower buds (before flower), flag leaves (after flower), flowers, milk seeds
Cellular locationnucleus
    ABclonal:Western blot - IPA1 Rabbit pAb (A17556)}

    Western blot - IPA1 Rabbit pAb (A17556)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using IPA1 Rabbit pAb (A17556) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 600s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)