제품 > 항체 > 다중클론항체(pAb)

IPO8 Rabbit pAb (A14679)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - IPO8 Rabbit pAb (A14679)

Western blot analysis of various lysates using IPO8 Rabbit pAb (A14679) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - IPO8 Rabbit pAb (A14679)

Immunofluorescence analysis of C6 cells using IPO8 Rabbit pAb (A14679) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - IPO8 Rabbit pAb (A14679)

Immunofluorescence analysis of HeLa cells using IPO8 Rabbit pAb (A14679) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - IPO8 Rabbit pAb (A14679)

Immunofluorescence analysis of L929 cells using IPO8 Rabbit pAb (A14679) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameIPO8 Rabbit pAb
Catalog No.A14679
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. This protein binds to the nuclear pore complex and, along with RanGTP and RANBP1, inhibits the GAP stimulation of the Ran GTPase. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 878-1037 of human IPO8 (NP_006381.2).
SequenceTRQLVNREDRSKAEKADMEENEEISSDEEETNVTAQAMQSNNGRGEDEEEEDDDWDEEVLEETALEGFSTPLDLDNSVDEYQFFTQALITVQSRDAAWYQLLMAPLSEDQRTALQEVYTLAEHRRTVAEAKKKIEQQGGFTFENKGVLSAFNFGTVPSNN
Gene ID
Swiss Prot
SynonymsVISS; RANBP8; IPO8
Calculated MW120kDa
Observed MW120kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, Jurkat, Mouse brain, Rat testis
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - IPO8 Rabbit pAb (A14679)}

    Western blot - IPO8 Rabbit pAb (A14679)

    Western blot analysis of various lysates using IPO8 Rabbit pAb (A14679) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Immunofluorescence - IPO8 Rabbit pAb (A14679)}

    Immunofluorescence - IPO8 Rabbit pAb (A14679)

    Immunofluorescence analysis of C6 cells using IPO8 Rabbit pAb (A14679) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - IPO8 Rabbit pAb (A14679)}

    Immunofluorescence - IPO8 Rabbit pAb (A14679)

    Immunofluorescence analysis of HeLa cells using IPO8 Rabbit pAb (A14679) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - IPO8 Rabbit pAb (A14679)}

    Immunofluorescence - IPO8 Rabbit pAb (A14679)

    Immunofluorescence analysis of L929 cells using IPO8 Rabbit pAb (A14679) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A14679? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)