제품 > 항체 > 다중클론항체(pAb)

IQSEC1 Rabbit pAb (A16725)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - IQSEC1 Rabbit pAb (A16725)

Western blot analysis of various lysates using IQSEC1 Rabbit pAb (A16725) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 5min.

Overview

Product nameIQSEC1 Rabbit pAb
Catalog No.A16725
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable protein kinase binding activity. Predicted to be involved in several processes, including positive regulation of focal adhesion disassembly; positive regulation of keratinocyte migration; and regulation of postsynaptic neurotransmitter receptor internalization. Located in nucleolus. Implicated in intellectual developmental disorder with short stature and behavioral abnormalities.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 180-380 of human IQSEC1 (NP_055684.3).
SequenceSFEGPEKVHSSYFEGKQVSVTNDGSQLGALVSPECGDLSEPTTLKSPAPSSDFADAITELEDAFSRQVKSLAESIDDALNCRSLHTEEAPALDAARARDTEPQTALHGMDHRKLDEMTASYSDVTLYIDEEELSPPLPLSQAGDRPSSTESDLRLRAGGAAPDYWALAHKEDKADTDTSCRSTPSLERQEQRLRVEHLPLL
Gene ID
Swiss Prot
SynonymsBRAG2; GEP100; IDDSSBA; ARFGEP100; ARF-GEP100; IQSEC1
Calculated MW108kDa
Observed MW108kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesmouse brain, rat brain
Cellular locationnucleolus, postsynaptic density, synaptic vesicle
ABclonal:Western blot - IQSEC1 Rabbit pAb (A16725)}

Western blot - IQSEC1 Rabbit pAb (A16725)

Western blot analysis of various lysates using IQSEC1 Rabbit pAb (A16725) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 5min.

* For research use only. Not for therapeutic or diagnostic purposes.