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ITPK1 Rabbit mAb (A20939)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - ITPK1 Rabbit mAb (A20939)

Western blot analysis of lysates from NCI-H460 cells, using ITPK1 Rabbit mAb (A20939) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - ITPK1 Rabbit mAb (A20939)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using ITPK1 Rabbit mAb (A20939) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ITPK1 Rabbit mAb (A20939)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using ITPK1 Rabbit mAb (A20939) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ITPK1 Rabbit mAb (A20939)

Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using ITPK1 Rabbit mAb (A20939) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameITPK1 Rabbit mAb
Catalog No.A20939
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2924
This gene encodes an enzyme that belongs to the inositol 1, 3, 4-trisphosphate 5/6-kinase family. This enzyme regulates the synthesis of inositol tetraphosphate, and downstream products, inositol pentakisphosphate and inositol hexakisphosphate. Inositol metabolism plays a role in the development of the neural tube. Disruptions in this gene are thought to be associated with neural tube defects. A pseudogene of this gene has been identified on chromosome X.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 315-414 of human ITPK1 (Q13572).
SequenceHIATVLQGQSTAMAATGDVALLRHSKLLAEPAGGLVGERTCSASPGCCGSMMGQDAPWKAEADAGGTAKLPHQRLGCNAGVSPSFQQHCVASLATKASSQ
Gene ID
Swiss Prot
SynonymsITRPK1; ITPK1
Calculated MW46kDa
Observed MW46kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesNCI-H460
Cellular locationApical plasma membrane, Cytosol.

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - ITPK1 Rabbit mAb (A20939)}

Western blot - ITPK1 Rabbit mAb (A20939)

Western blot analysis of lysates from NCI-H460 cells, using ITPK1 Rabbit mAb (A20939) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - ITPK1 Rabbit mAb (A20939)}

Immunohistochemistry - ITPK1 Rabbit mAb (A20939)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using ITPK1 Rabbit mAb (A20939) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ITPK1 Rabbit mAb (A20939)}

Immunohistochemistry - ITPK1 Rabbit mAb (A20939)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using ITPK1 Rabbit mAb (A20939) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ITPK1 Rabbit mAb (A20939)}

Immunohistochemistry - ITPK1 Rabbit mAb (A20939)

Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using ITPK1 Rabbit mAb (A20939) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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