제품 > 항체 > 단일클론항체(mAb)

Importin 9 / RANBP9 Rabbit mAb (A20901)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Importin 9 / RANBP9 Rabbit mAb (A20901) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Importin 9 / RANBP9 Rabbit mAb (A20901)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Importin 9 / RANBP9 Rabbit mAb (A20901) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product name	Importin 9 / RANBP9 Rabbit mAb
Catalog No.	A20901
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC2864

Background

Enables nuclear import signal receptor activity. Involved in protein import into nucleus. Located in cytoplasm.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Importin 9 / RANBP9 (Q96P70).
Sequence	LEVTEEFGVHLAELTVDPQGALAIRQLASVILKQYVETHWCAQSEKFRPPETTERAKIVIRELLPNGLRESISKVRSSVAYAVSAIAHWDWPEAWPQLFNL
Gene ID
Swiss Prot
Synonyms	Imp9; Importin 9 / RANBP9
Calculated MW	116kDa
Observed MW	116kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	HeLa, Jurkat, U-251MG, Mouse testis, Rat testis, Rat brain, Mouse brain
Cellular location	Cytoplasm, Nucleus
Customer validation	WB(Mus musculus)