Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat
Western blot analysis of various lysates using Integrin alpha 1/CD49a Rabbit mAb (A26489) at 1:250000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Confocal imaging of Neuro-2a cells using Integrin alpha 1/CD49a Rabbit mAb (A26489, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of paraffin-embedded Mouse lung tissue using Integrin alpha 1/CD49a Rabbit mAb (A26489, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
| Product name | Integrin alpha 1/CD49a Rabbit mAb |
|---|---|
| Catalog No. | A26489 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC68866 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 29-1141 of mouse Integrin alpha 1/CD49a (NP_001028400.2). |
|---|---|
| Sequence | FNVDVKNSMSFSGPVEDMFGYTVQQYENEEGKWVLIGSPLVGQPKARTGDVYKCPVGRERSMPCVKLDLPVNTSIPNVTEIKENMTFGSTLVTNPKGGFLACGPLYAYRCGHLHYTTGICSDVSPTFQVVNSFAPVQECSTQLDIVIVLDGSNSIYPWESVTAFLNDLLKRMDIGPKQTQVGIVQYGANVTHEFNLNKYSSTEEVLVAANKIGRRGGLQTMTALGIDTARKEAFTEARGARRGVKKVMVIVTDGESHDNYRLKQVIQDCEDENIQRFSIAILGHYNRGNLSTEKFVEEIKSIASEPTEKHFFNVSDELALVTIVKALGERIFALEATADQSAASFEMEMSQTGFSAHYSQDWVMLGAVGAYDWNGTVVMQKANQIVIPHNTTFQTEPTKMNEPLASYLGYTVNSATIPGDVLYIAGQPRYNHTGQVVIYKMEDGDVNILQTLSGEQIGSYFGSVLTTIDIDKDSYTDLLLVGAPMYMGTEKEEQGKVYVYAVNQTRFEYQMSLEPIKQTCCSSLKDNSCTKENKNEPCGARFGTAVAAVKDLNVDGFNDVVIGAPLEDDHAGAVYIYHGSGKTIRKEYAQRIPSGGDGKTLKFFGQSIHGEMDLNGDGLTDVTIGGLGGAALFWARDVAVVKVTMNFEPNKVNIQKKNCRVEGKETVCINATMCFHVKLKSKEDSVYEADLQYRVTLDSLRQISRSFFSGTQERRIQRNLTVRESECIRHSFYMLDKHDFQDSVRVTLDFNLTDPENGPVLDDALPNSVHGHIPFAKDCGNKERCVSDLTLDVSTTEKNLLIVRSQNDKFNVSLTVKNKGDSAYNTRTVVQYSPNLIFSGIEEIQKDSCESNQNITCRVGYPFLRTGDVVNFKIIFQFNTSHLSENAIIHLSATSDSEEPLESLYDNEVNISIPVKYEVGLQFYSSASEHHISVAANETVPELINSTKDIGDEINVFYTIRKRGHFPMPELRLAISFPNLTSDGYPVLYPTGWSSSDNVNCRPRSLEDPLGINSGKKMTISKSEVLKRGTIQDCSTCKIATITCHLLPSDVSQVNVSLILWKPTFIKAHFSSLNLTIRGELQSENSSLTLSSSNRKRELAIQISKDGLPGRVP |
| Gene ID | |
| Swiss Prot | |
| Synonyms | Vla1; CD49A; E130012M19Rik |
| Calculated MW | 131kDa |
| Observed MW | 200kDa |
| Reactivity | Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunofluorescence |
| Positive samples | Mouse kidney, Rat lung, |
| Cellular location | Membrane. |
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