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JAG1 Rabbit pAb (A12754)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - JAG1 Rabbit pAb (A12754)

Western blot analysis of extracts of Mouse brain, using JAG1 antibody (A12754) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameJAG1 Rabbit pAb
Catalog No.A12754
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter is involved in signaling processes. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 34-180 of human JAG1 (NP_000205.1).
SequenceQFELEILSMQNVNGELQNGNCCGGARNPGDRKCTRDECDTYFKVCLKEYQSRVTAGGPCSFGSGSTPVIGGNTFNLKASRGNDRNRIVLPFSFAWPRSYTLLVEAWDSSNDTVQPDSIIEKASHSGMINPSRQWQTLKQNTGVAHFE
Gene ID
Swiss Prot
SynonymsAGS; AHD; AWS; HJ1; AGS1; DCHE; CD339; JAGL1; CMT2HH
Calculated MW134kDa
Observed MW180kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse brain
Cellular locationMembrane, Single-pass type I membrane protein
ABclonal:Western blot - JAG1 Rabbit pAb (A12754)}

Western blot - JAG1 Rabbit pAb (A12754)

Western blot analysis of extracts of Mouse brain, using JAG1 antibody (A12754) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

재조합 단백질 (1)

ELISA 키트 (1)

Secondary Antibodies (26)