제품 > 항체 > 다중클론항체(pAb)

JAG2 Rabbit pAb (A14247)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - JAG2 Rabbit pAb (A14247)

Western blot analysis of lysates from Mouse heart, using JAG2 Rabbit pAb (A14247) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 20s.

ABclonal:Immunofluorescence - JAG2 Rabbit pAb (A14247)

Immunofluorescence analysis of C6 cells using JAG2 Rabbit pAb (A14247) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - JAG2 Rabbit pAb (A14247)

Immunofluorescence analysis of L929 cells using JAG2 Rabbit pAb (A14247) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameJAG2 Rabbit pAb
Catalog No.A14247
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development. Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions. The protein encoded by this gene is one of several ligands that activate Notch and related receptors. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 24-200 of human JAG2 (NP_002217.3).
SequenceARPMGYFELQLSALRNVNGELLSGACCDGDGRTTRAGGCGHDECDTYVRVCLKEYQAKVTPTGPCSYGHGATPVLGGNSFYLPPAGAAGDRARARARAGGDQDPGLVVIPFQFAWPRSFTLIVEAWDWDNDTTPNEELLIERVSHAGMINPEDRWKSLHFSGHVAHLELQIRVRCDE
Gene ID
Swiss Prot
SynonymsHJ2; SER2; LGMDR27; JAG2
Calculated MW133kDa
Observed MW150kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse heart
Cellular locationMembrane, Single-pass type I membrane protein

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ABclonal:Western blot - JAG2 Rabbit pAb (A14247)}

Western blot - JAG2 Rabbit pAb (A14247)

Western blot analysis of lysates from Mouse heart, using JAG2 Rabbit pAb (A14247) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 20s.
ABclonal:Immunofluorescence - JAG2 Rabbit pAb (A14247)}

Immunofluorescence - JAG2 Rabbit pAb (A14247)

Immunofluorescence analysis of C6 cells using JAG2 Rabbit pAb (A14247) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - JAG2 Rabbit pAb (A14247)}

Immunofluorescence - JAG2 Rabbit pAb (A14247)

Immunofluorescence analysis of L929 cells using JAG2 Rabbit pAb (A14247) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.