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JARID2 Rabbit mAb (A9614)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - JARID2 Rabbit mAb (A9614)

Western blot analysis of extracts of various cell lines, using JARID2 Rabbit mAb (A9614) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - JARID2 Rabbit mAb (A9614)

Western blot analysis of extracts of various cell lines, using JARID2 Rabbit mAb (A9614) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameJARID2 Rabbit mAb
Catalog No.A9614
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1658
This gene encodes a Jumonji- and AT-rich interaction domain (ARID)-domain-containing protein. The encoded protein is a DNA-binding protein that functions as a transcriptional repressor. This protein interacts with the Polycomb repressive complex 2 (PRC2) which plays an essential role in regulating gene expression during embryonic development. This protein facilitates the recruitment of the PRC2 complex to target genes. Alternate splicing results in multiple transcript variants. Mutations in this gene are associated with chronic myeloid malignancies.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human JARID2 (Q92833).
SequenceFEEGPSRKRPRLQAQRKFAQSQPNSPSTTPVKIVEPLLPPPATQISDLSKRKPKTEDFLTFLCLRGSPALPNSMVYFGSSQDEEEVEEEDDETEDVKTATN
Gene ID
Swiss Prot
SynonymsJMJ; DIDDF
Calculated MW139kDa
Observed MW150kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHT-29, Jurkat, Mouse lung, Mouse brain, Rat brain
Cellular locationNucleus
Customer validation

IF(Sus scrofa)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - JARID2 Rabbit mAb (A9614)}

    Western blot - JARID2 Rabbit mAb (A9614)

    Western blot analysis of extracts of various cell lines, using JARID2 Rabbit mAb (A9614) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - JARID2 Rabbit mAb (A9614)}

    Western blot - JARID2 Rabbit mAb (A9614)

    Western blot analysis of extracts of various cell lines, using JARID2 Rabbit mAb (A9614) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A9614? Please let us know so that we can cite the reference in this datasheet.

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