제품 > 항체 > 다중클론항체(pAb)

JMJD4 Rabbit pAb (A7992)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - JMJD4 Rabbit pAb (A7992)

Western blot analysis of various lysates, using JMJD4 antibody (A7992) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - JMJD4 Rabbit pAb (A7992)

Immunofluorescence analysis of PC-12 cells using JMJD4 Rabbit pAb (A7992) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameJMJD4 Rabbit pAb
Catalog No.A7992
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables 2-oxoglutarate-dependent dioxygenase activity. Involved in positive regulation of translational termination and protein hydroxylation. Located in cytoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-463 of human JMJD4 (NP_075383.2).
SequenceMRAGPEPQALAGQKRGALRLLVPRLVLTVSAPAEVRRRVLRPVLSWMDRETRALADSHFRGLGVDVPGVGQAPGRVAFVSEPGAFSYADFVRGFLLPNLPCVFSSAFTQGWGSRRRWVTPAGRPDFDHLLRTYGDVVVPVANCGVQEYNSNPKEHMTLRDYITYWKEYIQAGYSSPRGCLYLKDWHLCRDFPVEDVFTLPVYFSSDWLNEFWDALDVDDYRFVYAGPAGSWSPFHADIFRSFSWSVNVCGRKKWLLFPPGQEEALRDRHGNLPYDVTSPALCDTHLHPRNQLAGPPLEITQEAGEMVFVPSGWHHQVHNLDDTISINHNWVNGFNLANMWRFLQQELCAVQEEVSEWRDSMPDWHHHCQVIMRSCSGINFEEFYHFLKVIAEKRLLVLREAAAEDGAGLGFEQAAFDVGRITEVLASLVAHPDFQRVDTSAFSPQPKELLQQLREAVDAAAAP
Gene ID
Swiss Prot
SynonymsJMJD4
Calculated MW52kDa
Observed MW65kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesSK-BR-3, PC-3, NCI-H460
Cellular locationcytoplasm, nucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - JMJD4 Rabbit pAb (A7992)}

    Western blot - JMJD4 Rabbit pAb (A7992)

    Western blot analysis of various lysates, using JMJD4 antibody (A7992) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunofluorescence - JMJD4 Rabbit pAb (A7992)}

    Immunofluorescence - JMJD4 Rabbit pAb (A7992)

    Immunofluorescence analysis of PC-12 cells using JMJD4 Rabbit pAb (A7992) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)

    HRP-conjugated Goat anti-Rabbit IgG (H+L)
    AS014

    WBDBIHC-PELISA

    HRP-conjugated Goat anti-Rabbit IgG (H+L)

    FITC-conjugated Goat anti-Rabbit IgG (H+L)
    AS011

    IF/ICCFC

    FITC-conjugated Goat anti-Rabbit IgG (H+L)

    Cy3-conjugated Goat anti-Rabbit IgG (H+L)
    AS007

    IF/ICCFC

    Cy3-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
    AS053

    IF/ICCFC

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain
    AS061

    WBIP

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain

    HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
    AS063

    WB

    HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

    ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
    AS039

    IF/ICCFC

    ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

    Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
    AS040

    IHC-PIF/ICCFC

    Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
    AS060

    IF/ICCFC

    ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

    Goat Anti-Rabbit IgG(H+L)
    AS070

    WBELISACUT&Tag

    Goat Anti-Rabbit IgG(H+L)

    HRP-conjugated Donkey anti-Rabbit IgG (H+L)
    AS038

    WBIHC-P

    HRP-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
    AS035

    IF/ICCFC

    ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
    AS073

    IF/ICCFC

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
    AS058

    IF/ICCFC

    ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

    FITC-conjugated Donkey anti-Rabbit IgG (H+L)
    AS042

    IF/ICCFC

    FITC-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
    AS056

    IF/ICCFC

    ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

    AP-conjugated Goat anti-Rabbit IgG (H+L)
    AS009

    WB

    AP-conjugated Goat anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG (H+L)
    AS079

    WB

    HRP-conjugated Mouse anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
    AS126

    IPELISA

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

    PE Mouse anti-Rabbit IgG, light chain specific mAb
    AS129

    FC

    PE Mouse anti-Rabbit IgG, light chain specific mAb

    HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
    AS122

    WBELISA

    HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

    Rabbit Anti-Chicken IgY (H+L)
    AS090

    WB

    Rabbit Anti-Chicken IgY (H+L)

    Rabbit Anti-Chicken IgY Fab
    AS088

    WB

    Rabbit Anti-Chicken IgY Fab

    FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
    AS083

    IF/ICCFC

    FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

    Rabbit Anti-Chicken IgY (H+L)
    AS082

    WB

    Rabbit Anti-Chicken IgY (H+L)

    ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
    AS072

    IF/ICCFC

    ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)